RYUL KIM^1,#, JOO KYUNG PARK^2,#, MINSUK KWON³, MINAE AN⁴, JUNG YONG HONG¹, JOON OH PARK¹, SUNG HEE LIM^1,*, SEUNG TAE KIM^1,*

Oncology Research, Vol.33, No.1, pp. 57-65, 2025, DOI:10.32604/or.2024.049054 - 20 December 2024

Abstract Background: Immune checkpoint inhibitors (ICIs) are effective in a subset of patients with metastatic solid tumors. However, the patients who would benefit most from ICIs in biliary tract cancer (BTC) are still controversial. Materials and methods: We molecularly characterized tissues and blood from 32 patients with metastatic BTC treated with the ICI pembrolizumab as second-line therapy. Results: All patients had microsatellite stable (MSS) type tumors. Three of the 32 patients achieved partial response (PR), with an objective response rate (ORR) of 9.4% (95% confidence interval [CI], 2.0–25.2) and nine showed stable disease (SD), exhibiting a disease… More >

Yuan Wang¹, Tianle Zhang¹, Xinfeng Zeng¹, Jiale Liu¹, Siyu Li¹, Siyu Yang¹, Shengnan Zhao¹, Abdullah Shah², Muhammad Saif Ullah², Guohui Qi^1,*, Peng Jia^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3331-3346, 2024, DOI:10.32604/phyton.2024.059402 - 31 December 2024

Abstract LONELY GUY (LOG) is a pivotal cytokinin-activating enzyme that plays an important role in plant growth, development, and stress responses. Walnut (Juglans regia), an important woody oilseed species, has not yet undergone systematic identification of its LOG gene family. In this study, we identified 17 JrLOG genes in the walnut genome, which are unevenly distributed across 11 chromosomes. JrLOG gene expansion was primarily driven by gene duplication, along with purifying selection. Members of the JrLOG family were categorized into five groups, each exhibiting analogous gene structures, featured motifs, and conserved domains. Transcriptome and quantitative real-time PCR analyses More >

Xiaodong Shi^*, Tingyu Wang, Sui Ai, Jiasi Li

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2571-2581, 2024, DOI:10.32604/phyton.2024.056606 - 30 October 2024

Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract

Jiayu Luo^1,#, Jianfeng Liu^1,#, Zuoxiang Sun¹, Pengpeng Shao¹, Danyang Wang², Jianzhong Yao³, Binzhou Li², Lijun Dong¹, Dandan Cao^1,4,*, Jianrong Wei^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2167-2188, 2024, DOI:10.32604/phyton.2024.052858 - 30 September 2024

Abstract Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars. However, one poplar species, Populus deltoides, has strong resistance to Anoplophora glabripennis infestation, the underlying defense mechanisms against Anoplophora glabripennis are poorly understood. Secondary metabolites play a crucial role in plants to combat biological stress. Here, based on transcriptome and metabolome, we demonstrated that the mechanisms for responses to mechanical damage and insect infestation were different. The degree of reactions to adult groove production, larval incubation, and larval frass production was not identical. In addition, the potential genes with insect resistance activity were identified. Predominant differentially More >

Abdul Karim Amin¹, Yan He¹, Xianglong Wang¹, Pengwei Li¹, Muhammad Ahmad Hassan², Mohammad Yousof Soltani³, Yiling Zhang¹, Mohammad Alem Amin⁴, Ahmad Shah Ahmadzai⁵, Yajing Liu^1,3,*, Songhu Wang^1,3,*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 2075-2093, 2024, DOI:10.32604/phyton.2024.054641 - 30 August 2024

Abstract Tomato (Solanum lycopersicum) is a perishable fruit because of its fast water loss and susceptibility to pathogens in the post-harvest stage, which leads to huge economic losses every year. In this study, firstly from 19 tomato cultivars, we screened out two cultivars, Riogrand and SalarF1, having long and short shelf-life spans, respectively. Secondly, shelf-life analysis was carried out for both cultivars at room temperature. Results exhibited that Riogrand showed higher firmness and less weight loss than SalarF1. The ethylene production was higher in SalarF1, compared with Riogrand during post-harvest storages. We performed transcriptomic analysis of both… More >

Yolotzin Apatzingán Palomino-Hermosillo¹, Ángel Elpidio Díaz-Jasso², Rosendo Balois-Morales¹, Verónica Alhelí Ochoa-Jiménez^1,3, Pedro Ulises Bautista-Rosales¹, Guillermo Berumen-Varela^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1717-1732, 2024, DOI:10.32604/phyton.2024.052216 - 30 July 2024

Abstract Soursop (Annona muricata L.) is a tropical fruit highly valued for its unique flavor, nutritional value, and health-promoting properties. The ripening process of soursop involves complex changes in gene expression and metabolite accumulation, which have been studied using various omics technologies. Transcriptome analysis has provided insights into the regulation of key genes involved in ripening, while metabolic compound analysis has revealed the presence of numerous bioactive compounds with potential health benefits. However, the integration of transcriptome and metabolite compound data has not been extensively explored in soursop. Therefore, in this paper, we present a comprehensive analysis… More >

Mingchuan Yang¹, Jia Zheng², Wenhui Yu¹, Yanghua Li², Yali Wang¹, Zilu Zhang¹, Zhenhui Kang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1503-1521, 2024, DOI:10.32604/phyton.2024.051718 - 30 July 2024

Abstract Sorghum is not only an important bio-energy crop but also a vital raw material for brewing. Exogenous copper affects the growth and metabolism of crops in specific ways. This study identified 8475 differentially expressed genes (DEGs) by high-throughput transcriptome sequencing in the sorghum cultivar ‘Jinnuoliang 2’ after 24 h of treatment with 10 mM CuSO. Using GO analysis, 476 genes were functionally annotated, which were mainly related to catabolism and biosynthetic processes. Additionally, 90 pathways were annotated by employing the KEGG analysis. Among them, glutathione metabolism and peroxisome were induced, while photosynthesis, photosynthesis-antenna protein, and… More >

Fangye Liu^1,#, Jiaping Qu^1,#, Yajun Li¹, Jiawei Fan¹, Yumeng Cui¹, Jingwen Wu¹, Xueqi Gu¹, Xia Li^2,*, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1599-1621, 2024, DOI:10.32604/phyton.2024.051386 - 30 July 2024

Abstract Chrysanthemum × morifolium is a horticultural crop which plays a vital role in the flower industry with significant economic value and has a cultivation history of over three thousand years in China. The accumulation of anthocyanins is always affected by light. Here, we revealed that anthocyanin accumulation is highly dependent on light in ‘2021135’ genotype chrysanthemum, while it is light-independent in ‘2001402’ genotype chrysanthemum. However, no literature has been reported regarding the non-photosensitive chrysanthemum in anthocyanins light-independent synthesis pathways. Through the phenotype analysis of 44 F generations, we found that light-independence is a dominant trait which… More >

Yong Zhao^1,#, Yaxuan Jiang^3,#, Li Wen¹, Rui Luo², Guorui Li², Jianjun Di², Mingda Yin², Zhiyan Wang², Fenglan Huang^2,4,5,6,7,*, Fanjuan Meng^3,*

Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 713-723, 2024, DOI:10.32604/phyton.2024.047657 - 29 April 2024

Abstract The yield of castor is influenced by the type of inflorescence and the proportion of female flowers. However, there are few studies on the genetic mechanism involved in the development and differentiation of castor inflorescences. In this study, we performed transcriptomic analyses of three different phenotypes of inflorescences at the five-leaf stage. In comparison to the MI (complete pistil without willow leaves), 290 and 89 differentially expressed genes (DEGs) were found in the SFI (complete pistil with willow leaves) and the BI (monoecious inflorescence), respectively. Among the DEGs, 104 and 88 were upregulated in the… More >

JINGJING CHEN¹, DAN WANG², ZEQUN WANG², MENGYUAN HAN⁴, HOUQING YIN², WENTING ZHOU⁴, RIBAI YAN⁵, YAN PAN^2,3,*

Oncology Research, Vol.32, No.5, pp. 911-923, 2024, DOI:10.32604/or.2023.042384 - 23 April 2024

Abstract Photodynamic therapy (PDT) is a promising cancer treatment. This study investigated the antitumor effects and mechanisms of a novel photosensitizer meso-5-[ρ-diethylene triamine pentaacetic acid-aminophenyl]−10,15,20-triphenyl-porphyrin (DTP) mediated PDT (DTP-PDT). Cell viability, reactive oxygen species (ROS), and apoptosis were measured with a Cell Counting Kit-8 assay, DCFH-DA fluorescent probe, and Hoechst staining, respectively. Cell apoptosis- and autophagy-related proteins were examined using western blotting. RNA sequencing was used to screen differentially expressed mRNAs (DERs), and bioinformatic analysis was performed to identify the major biological events after DTP-PDT. Our results show that DTP-PDT inhibited cell growth and induced ROS… More > Graphic Abstract