Guan Z-J^1,3,4*, S-B Lu^2*,Y-L Huo⁵, B Guo³, Z-P Guan⁶, Y-H Wei³

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

Vargas-Bejarano E, V Méndez-Trujillo, JC Vázquez Angulo, D González-Mendoza, O Grimaldo Juarez

Phyton-International Journal of Experimental Botany, Vol.81, pp. 101-105, 2012, DOI:10.32604/phyton.2012.81.101

Abstract We performed the inoculation of transgenic cotton seedlings with Trichoderma sp. strain ICA 4 to evaluate its effects on the physiological development of seedlings. Inoculated seedlings of transgenic cotton had higher development than non-inoculated seedlings. The range of values for relative growth, doubling time, height and number of leaves (0.04 g/g d; 16.88 days; 17.16 cm and 4.2, respectively) were significantly higher that those observed in non-inoculated plants (0.03 g/g day; 24.38 days; 12.33 cm and 2.6, respectively). Regarding radical architecture, inoculated seedlings with Trichoderma strain ICA 4 showed a higher percentage of roots of 2^nd (46%), 3^rd (73%) and… More >

Guan Z-J^1,2,*, B Guo^1,*, Y-L Huo³, J-K Dai⁴, Y-H Wei¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051

Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >

MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI

BIOCELL, Vol.26, No.2, pp. 217-223, 2002, DOI:10.32604/biocell.2002.26.217

Abstract Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model”… More >

Mohammad UBAIDILLAH^1,2, Miswar FAPERTA^1,2, Kyung-Min KIM¹

BIOCELL, Vol.43, No.3, pp. 215-224, 2019, DOI:10.32604/biocell.2019.07549

Abstract Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison between non transgenic and transgenic rice harboring CaMsrB2 gene, which induces tolerance to abiotic stress, is important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and phytohormone levels under abiotic stress, to be closely related. The CaMsrB2 gene is related to the expression of JA and ABA hormones. Therefore, the level of expression of these genes and hormones was observed. The transcription levels… More >