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    ARTICLE

    Identification, Isolation and Characterization of GaCyPI Gene in Gossypium arboreum under Cotton Leaf Curl Virus Disease Stress

    Zunaira Sher1, Muhammad Umair Majid1, Sameera Hassan1, Fatima Batool1, Beenish Aftab1,2, Bushra Rashid1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1613-1632, 2021, DOI:10.32604/phyton.2021.016154

    Abstract Pakistan is facing the threat of Cotton Leaf Curl Virus (CLCuV) which is transmitted through whitefly to cotton crop. Molecular mechanism of leaf epicuticular wax protects the plants from different pathogens including insect attack and disease transmission. Objective of current study is the isolation and characterization of a wax related gene GaCyPI from Gossypium arboreum under CLCuV infection. A fragment of 475 bp was isolated from the total RNA and 3’ and 5’ RACE-PCR products were arranged by overlapping the extended sequences at both the ends. Deduced protein sequence of GaCyPI showed homology with Cyclophilin cis-trans isomerase gene of Gossypium… More >

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