CLAUDIA AGUILERA MERLO, ESTELA MUÑOZ, SUSANA DOMINGUEZ, MABEL FÓSCOLO*, LUIS SCARDAPANE, JUAN CARLOS DE ROSAS*
BIOCELL, Vol.29, No.3, pp. 243-251, 2005, DOI:10.32604/biocell.2005.29.243
Abstract Seasonal changes in the reproductive activity of the adult male viscacha (Lagostomus maximus maximus) were investigated during the annual reproductive cycle. Assays of heterologous in vitro binding between compatible gametes were used to evaluate the ability of viscacha spermatozoa to achieve primary binding during its annual reproductive cycle. Sperm were collected by mincing cauda epididymis in HECM-3 medium and the sperm concentration and motility were evaluated. Cumulus-free and zona-free oocytes obtained from superovulated hamsters were inseminated in vitro with capacitated sperm suspensions, incubated at 37ºC, 5% CO2 for 3 h, and then processed for studies by scanning electronic microscopy. Statistical… More >
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