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  • Open Access

    ARTICLE

    Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

    Quezada-Rivera JJ1, RE Soria-Guerra2, FS Pérez-Juárez2, L Martínez-González2, SE Valdés- Rodríguez3, NL Vasco-Méndez1, JF Morales-Domínguez1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 25-35, 2019, DOI:10.32604/phyton.2019.04549

    Abstract The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified… More >

  • Open Access

    ARTICLE

    Influences of ascorbic acid and gibberellic acid in alleviating effects of salinity in Petunia under in vitro

    Krupa-Małkiewicz M1, B Smolik2, M Sędzik2

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 15-23, 2019, DOI:10.32604/phyton.2019.04670

    Abstract Salinity is one of the abiotic stresses that limits the growth and productivity of many crops. A possible survival strategy for plant under saline conditions is to use compounds that could minimize the harmful effects of salt stress on the plant development. The objective of the presented study was to investigate the effect of exogenous ascorbic acid (ASA) with or without gibberellic acid (GA3) on key growth and biochemical parameters in two petunia cultivars ‘Prism Rose’ and ‘Prism White’ under saline (150 mM NaCl) and non-saline in vitro condition. Nodal cutting with an axillary buds were used as explants. Application… More >

  • Open Access

    ARTICLE

    First report of Phytopythium vexans causing the “Avocado sadness” in Michoacan, Mexico

    Hernández Pérez A1, E Cerna Chávez2, JC Delgado Ortiz3, M Beltrán Beache4, LM Tapia Vargas5, YM Ochoa Fuentes2

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 11-13, 2019, DOI:10.32604/phyton.2019.04608

    Abstract Mexico is the main producer, consumer and exporter of avocado in the world, being Michoacan the main producer state contributing more than 80% of the national production. There are phytopathogens that decimate the production causing the death of the tree. Root samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness, the sampling was carried out in four of the main avocado producing towns, in the state of Michoacan, Mexico. The isolation consisted in sowing root tissue in Petri dishes with V8®-PARPH culture medium, subsequently they were identified morphologically and for species… More >

  • Open Access

    REVIEW

    Active compounds of medicinal plants, mechanism for antioxidant and beneficial effects

    Orozco Montes F1, A Vázquez-Hernández2, B Fenton-Navarro1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 1-10, 2019, DOI:10.32604/phyton.2019.04525

    Abstract An increased interest in the antioxidant effects of medicinal plants has developed in recent years. Identifying antioxidant compounds present in medicinal plants and elucidating the mechanism by which they prevent oxidation have been the focus of the research community. We performed a systematic and exhaustive review aimed at analyzing the available data regarding the beneficial effects of secondary metabolites in plants. The result of this review is presented as a description of free radicals, as well as cellular and physiological oxidative stress, is provided. The origin and source of antioxidant compounds, and the cellular and molecular mechanism by which they… More >

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