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  • Open Access

    ARTICLE

    In vitro germination of Encyclia adenocaula (La Llave & Lex.) Schltr (Orchidaceae) seeds

    Ruíz1,2,* BC, CA Laguna2, ALG Iglesias3, A Damon4, HTNJ Marín5, RHS Azpíroz5, MJL Moreno1

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 203-215, 2008, DOI:10.32604/phyton.2008.77.203

    Abstract Encyclia adenocaula (La Llave & Lex.) Schltr. (Orchidaceae) is an epiphyte orchid, which uses oak (Quercus sp) trees as a natural host and is thus considered a non timber-yielding forest resource. Predation of this species is high because of the beauty of its flowers. The Official Mexican Norm (NOM-059-SEMARNAT-2001) reports it as an endemic endangered species in Mexico. The objective of this research was to (1) establish an effective protocol for in vitro germination of immature seeds of this species, and (2) determine the effect of the capsule maturity state on seed germination. The in vitro cultivation mediums were:… More >

  • Open Access

    ARTICLE

    Sampling and characterization of pepper chilli (Capsicum spp) in Tabasco, Mexico

    Castañón-Nájera2 G, L Latournerie-Moreno3, M Mendoza-Elos4, A Vargas-López5, H Cárdenas-Morales6

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 189-202, 2008, DOI:10.32604/phyton.2008.77.189

    Abstract A morphological characterization in situ of Capsicum spp was made in 13 localities in the state of Tabasco, México, during 2004 and 2005. The objective was to sample and identify different morphotypes of chilli pepper which grow under wild and cultivated conditions. Eleven chilli pepper morphotypes were found. Most of them were wild (Amashito, Corazón de pollo, Muela, Garbanzo, Garbanzo raro and Desconocido) and corresponded to C. annuum. Another morphotype was half wild (Picopaloma) and corresponded to C. frutescens. The remaining morphotypes corresponded to C. annuum and C. chinenese. Data were analyzed with multivariate statistics. Principal component analysis (PCA) indicated that… More >

  • Open Access

    ARTICLE

    Saturation of a durum wheat genetic map and detection of QTL associated to lipoxygenase activity

    Picca1 A, P Roncallo1, A Carrera1, G Cervigni1, R. Miranda2, V Echenique1

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 175-188, 2008, DOI:10.32604/phyton.2008.77.175

    Abstract The aims of this work were (1) the saturation of a linkage map of durum wheat using AFPLs and RAPDs, (2) mapping of QTL related to lipoxygenase activity and (3) estimation of its usefulness in marker-assisted selection to increase pasta colour. A mapping population of 83 recombinant lines (RILs) derived from the cross between durum wheat Kofa and UC1113 (Triticum turgidum L. var. durum) was evaluated for lipoxygenase activity during two growing seasons. It was used to generate a genetic map. Forty four AFLPs, 9 RAPDs, 2 isoenzymes and 1 storage protein were mapped onto a previous More >

  • Open Access

    ARTICLE

    Effect of heat conditioning on chilling injury susceptibility of Persian lime (Citrus latifolia Tanaka)

    Bosquez Molina1 E, J Domínguez Soberanes1, L Perez Flores2, S Bautista Baños3, F Díaz de León Sánchez2, F Rivera-Cabrera2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 161-174, 2008, DOI:10.32604/phyton.2008.77.161

    Abstract Cold storage is one of the most important technologies used to maintain the postharvest quality of many horticultural commodities. However, cold storage is restricted for Persian lime because of its susceptibility to chilling injury. The objective of this study was to evaluate the effects of high temperature conditioning on the susceptibility to chilling injury in Persian lime (Citrus latifolia Tanaka). It was harvested in Martínez de la Torre, Veracruz, Mexico, and stored at different refrigeration temperatures. After harvesting, fruits received the following treatments: (1) hot water conditioning (53 °C during 3 min); (2) hot air conditioning More >

  • Open Access

    ARTICLE

    Reproduction of Pavonia cymbalaria (Malvaceae), a native species with ornamental potential

    Torres, YA1, MA Long2, SM Zalba1

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 151-160, 2008, DOI:10.32604/phyton.2008.77.151

    Abstract Pavonia cymbalaria A. St.-Hil. & Naudin (Malvaceae) is a native species of the southern mountains in the Province of Buenos Aires. Its morphological characteristics become it as a potential candidate for ornamental use. Crop requirements and seed germination were evaluated for this species in this study. Different treatments were applied to 640 mericarps: removal of carpel residues, mechanical scarification or exposition of mericarps and nude seeds to cold conditions. An untreated control was used for each of these treatments. Germination only occurred on nude seeds; this implies the existence of an extrinsic dormancy imposed by the… More >

  • Open Access

    ARTICLE

    Effect of four vermicomposts on growth of Digitaria eriantha plants

    Giulietti1 AL, OM Ruiz1, HE Pedranzani1, O Terenti1,2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 137-149, 2008, DOI:10.32604/phyton.2008.77.137

    Abstract In this paper, the effects of four vermicomposts are analyzed on a variety of growth and development parameters of Digitaria eriantha cv. Mejorada INTA. The vermicomposts used originated from cow, horse, goat and hen feaces combined with pure soil in the following proportions: T1: 0% worm humus + 100% soil; T2: 50% worm humus + 50% soil, and T3: 100% worm humus + 0% soil. Digitaria eriantha seeds were sown in trays which were kept under controlled light and moisture conditions. After the third week of treatment, the number of germinated seeds, and both leaf and More >

  • Open Access

    ARTICLE

    Diagnosis of the microbiological quality of fruits and vegetables in Chihuahua, Mexico

    Ávila-Quezada1* G, E Sánchez1, E Muñoz1, LR Martínez2, E Villalobos2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 129-136, 2008, DOI:10.32604/phyton.2008.77.129

    Abstract We determined the microbiological quality of the most important fruits and vegetables in Chihuahua State (Mexico) to know the potential risks and apply prevention strategies prior to their consumption. Samples of jalapeño, bell and serrano pepper, cantaloupe, saladet and grape tomato, peach and Golden and Starkimson apple and of well, channel, irrigation and drinking water, as well as that for washing in packing were analyzed to determine Salmonella spp., Escherichia coli, total and fecal coliforms, fungi and yeasts. Determinations were made following the methodology indicated by the Mexican regulations (NOM-111-SSA1-1994). Sampling was made in the whole More >

  • Open Access

    ARTICLE

    Quantitative measures of leaf epidermal cells as a taxonomic and phylogenetic tool for the identification of Stanhopea species (Orchidaceae)

    Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

    Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed More >

  • Open Access

    ARTICLE

    Biological activity of phototoxic compounds in Bidens squarrosa H.B.K. (Asteraceae)

    Pérez-Amador1 MC, V Muñoz Ocotero1, F García Jiménez2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 103-111, 2008, DOI:10.32604/phyton.2008.77.103

    Abstract Phototoxic compounds (polyacetylenes and thiophenes) and their biological activity were determined on Bidens squarrosa plants by measuring their antibacterial and anti-inflammatory potential. Phototoxic compounds were extracted with hexane, characterized by TLC, and their presence confirmed by U.V. spectrum of their extracts and bactericidal potential. Antibacterial activity of the hexane, ethyl acetate and methanol extracts was measured against Bacillus subtilis and Escherichia coli, and their antiinflammatory potential was determined by the mouse ear edema test. More >

  • Open Access

    ARTICLE

    Plant micropropagation in experimental lines of forage maize coming from crossing Zea mays L. and Zea diploperennis Iltis, Doebley and Guzmán

    Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

    Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the More >

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