Vol.89, No.2, 2020, pp.229-251, doi:10.32604/phyton.2020.10022
OPEN ACCESS
ARTICLE
Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley
  • Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue*, Xiaoqin Zhang*
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China
* Corresponding Authors: Dawei Xue. Email: dwxue@hznu.edu.cn; Xiaoqin Zhang. Email: xiaoqinzhang@163.com
Received 05 February 2020; Accepted 29 February 2020; Issue published 22 April 2020
Abstract
The F-box protein-encoding gene family plays an essential role in plant stress resistance. In present study, 126 non-redundant F-box genes were identified in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165– 887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana (At) was used to classify barley F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs. In total, 124 F-box genes were unevenly distributed on 7 chromosomes; another 2 genes have not been anchored yet. The gene structure analysis revealed high variability in the number of exons and introns in F-box genes. Comprehensive analysis of expression profiles and phylogenetic tree analysis, a total of 12 F-box genes that may be related to stress tolerance in barley were screened. Of the 12 detected F-box genes, 8 and 10 were upregulated after drought and salt stress treatments, respectively, using quantitative real-time polymerase chain reaction (qRT-PCR). This study is the first systematic analysis conducted on the F-box gene family in barley, which is of great importance for clarifying this family’s bioinformatic characteristics and elucidating its function in barley stress resistance. These results will serve as a theoretical reference for subsequent research on molecular regulation mechanisms, genetic breeding, and improvement.
Keywords
Drought and salt stress; barley; expression analysis; F-box gene family; phylogenetic analysis
Cite This Article
Zhang, L., Wang, S., Chen, Y., Dong, M., Fang, Y. et al. (2020). Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley. Phyton-International Journal of Experimental Botany, 89(2), 229–251.