Open Access

ARTICLE

Functional interleukin-10 promoter variants in coronary artery disease patients in Tunisia

Sonia Ben-Hadj-Khalifa¹, Lakhdar Ghazouani¹, Nesrine Abboud¹, Ali Ben-Khalfallah², Fatma Annabi², Faouzi Addad³, Wassim Y. Almawi⁴, Touhami Mahjoub¹

1 Research Unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Monastir
2 Menzel Bourguiba Hospital Centre, Bizerte
3 CHU Fattouma Bourguiba, Monastir, Tunisia
4 College of Medicine and Medical Sciences, Arabian Gulf. University, Manama, Bahrain

* Corresponding Author: W.Y. Almawi,

European Cytokine Network 2010, 21(2), 136-141. https://doi.org/10.1684/ecn.2010.0194

Accepted 02 April 2010;

Abstract

Objectives. The contribution of interleukin (IL)-10 promoter variants -1082G/A, -819C/T, and -592C/A to the risk of coronary artery disease (CAD) was investigated in 291 CAD patients and 291 age- and gender-matched control subjects. Methods and results. IL-10 genotyping was performed using PCR-allele-specific amplification (PCR-ASA). Regression analysis was employed in assessing the contribution of the IL-10 variants to the overall CAD risk. A higher frequency of the -592A allele (p = 0.004), but not the -1082A (p = 0.828) or -819T (p = 0.952) alleles, was seen in CAD patients. A higher frequency of -592C/A (p = 0.011), and a lower frequency of -592C/C (p = 0.015) genotypes was noted in patients compared to healthy controls. Regression analysis demonstrated an association of -592C/A [OR (95% CI) = 1.82 (1.02-3.23)] and -592A/A [OR (95% CI) = 3.33 (1.27-9.09)] genotypes with 1-artery disease. Haplotype analysis revealed that none of the eight possible IL-10 haplotypes was associated with CAD or with the severity of CAD, and was confirmed by multivariate regression analysis, after adjusting for a number of confounders (smoking, systolic and diastolic blood pressure, hypertension, diabetes, glucose, cholesterol, and triglycerides). Conclusions. Our results suggest that the -592C/A, more so than the -1082G/A or the -819C/T IL-10 promoter variant alleles, may be considered to be a risk factor for CAD in Tunisians.

---

Keywords

---