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Freely Available Tool (FAT) for automated quantification of lipid droplets in stained cells

Diego Masone1,2, Aldana D. Gojanovich1, Yesica R. Frontini-Lopez1, Samanta DEL Veliz1, Marina Uhart1, Diego M. Bustos1,3*
1 Laboratorio de Integración de Señales Celulares. IHEM-CONICET, Universidad Nacional de Cuyo Mendoza, Argentina.
2 Facultad de Ingeniería, Universidad Nacional de Cuyo, Mendoza, Argentina.
3 Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.
* Address correspondence to: Diego M. Bustos,

BIOCELL 2017, 41(2-3), 55-58. https://doi.org/10.32604/biocell.2017.41.055

Abstract

In this study, we propose an automatic procedure for digital image processing. We describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in culture. Prospectively, the lipid droplets detection method described in this work could be applied to static or time-lapse data, collected with a simple visible light or fluorescence microscopy equipment. Fully automated algorithms were implemented in Octave, a freely available scientific package.

Keywords

Adipogenesis, 3T3-L1 cells, human stem cells, automatic image processing.

Cite This Article

Masone, D., Gojanovich, A. D., Frontini-Lopez, Y. R., Veliz, S. D., Uhart, M. et al. (2017). Freely Available Tool (FAT) for automated quantification of lipid droplets in stained cells. BIOCELL, 41(2-3), 55–58.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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