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ARTICLE
Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of Pisum sativum (L.) cells germinated in Euryops arabicus (Steud. ex Jaub. & Spach) water extract
Mahmoud MOUSTAFA1,2, Saad ALAMRI1, Hoida ZAKI2, Naglaa LOUTFY2, Tarek TAHA3, Ali SHATI1, Mohamed ALKAHTANI1, Sajda SIDDIQI1
1
Department of Biology, College of Science, King Khalid University, 9004, Abha, Kingdom of Saudi Arabia (KSA)
2
Department of Botany, Faculty of Science, South Valley University, Qena, Egypt
3
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt
* Address correspondence to: Mahmoud MOUSTAFA,
BIOCELL 2019, 43(2), 81-88. https://doi.org/10.32604/biocell.2019.06231
Abstract
The present research reports of quick and marked changes induced by plant extract of
Euryops arabicus in the
gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (
Pisum
sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated
anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based
on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases,
cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E.
arabicus extracts
were sharply increased than those sown in distilled water. All the samples exhibited that the stems had more amount
from apyrases, cytoskeletal proteins, amino acids and ATPase and ADPase activities than primary leaves and primary
roots that were germinated either on E.
arabicus water extract or in distilled water. Based on the enzyme’s capability to
hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of
49-kDa apyrase and cytoskeletal proteins, E.
arabicus water extract had an important effect on plant germinations.
Keywords
Cite This Article
MOUSTAFA, M., ALAMRI, S., ZAKI, H., LOUTFY, N., TAHA, T. et al. (2019). Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of
Pisum sativum (L.) cells germinated in E
uryops arabicus (Steud. ex Jaub. & Spach) water extract.
BIOCELL, 43(2), 81–88.