Table of Content

Open Access iconOpen Access

ARTICLE

crossmark

Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

YUNZE XUAN1,#, BIN JIN1,#, SAYAN DEB DUTTA5,#, MENGMENG LIU2, ZAIXIAN SHEN1, XIWEN LIU3, YANG KANGJUAN4,*, LIM KI-TAEK5,*

1 Department of Stomatology, Affiliated Hospital of Yanbian University, Yanji, 133000, China
2 Department of Immunology and Molecular Microbiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, 08826, South Korea
3 Affiliated Hospital of Yanbian University, Yanji, 133000,
4 Department of Cell Biology and Medical Genetics, Yanbian University Medical College, Yanji, 133000, China
5 Department of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, South Korea

* Address correspondence to: Kangjuan Yang, email; Ki-Taek Lim, email
# These authors contributed equally to this work

(This article belongs to the Special Issue: Stem Cells, Protein Therapeutics, and Regenerative Medicine)

BIOCELL 2020, 44(3), 389-400. https://doi.org/10.32604/biocell.2020.09170

Abstract

Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay, Alizarin Red-S, and alkaline phosphatase staining, and real-time PCR. Notably, 300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs, indicating their osteogenic potential. Moreover, a significant enhancement of osteogenesis gene markers and proteins was observed. We also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast differentiation. These results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.

Keywords


Cite This Article

APA Style
XUAN, Y., JIN, B., DUTTA, S.D., LIU, M., SHEN, Z. et al. (2020). Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid. BIOCELL, 44(3), 389-400. https://doi.org/10.32604/biocell.2020.09170
Vancouver Style
XUAN Y, JIN B, DUTTA SD, LIU M, SHEN Z, LIU X, et al. Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid. BIOCELL . 2020;44(3):389-400 https://doi.org/10.32604/biocell.2020.09170
IEEE Style
Y. XUAN et al., "Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid," BIOCELL , vol. 44, no. 3, pp. 389-400. 2020. https://doi.org/10.32604/biocell.2020.09170

Citations




cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 2636

    View

  • 1805

    Download

  • 0

    Like

Share Link