Open Access
ARTICLE
Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Phragmites australis
ZHIHUI WANG1, YE YUAN2,*
1 College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319, China
2 Mudanjiang Tobacco Science Research Institute, Haerbin, 150000, China
* Address correspondence to: Ye Yuan,
(This article belongs to this Special Issue: )
BIOCELL 2020, 44(4), 731-736. https://doi.org/10.32604/biocell.2020.010575
Received 11 March 2020; Accepted 26 June 2020; Issue published 24 December 2020
Abstract
Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-
Enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway in microorganisms and
plants. The full-length
epsps cDNA sequence (
paepsps, Genebank: KY860582.1) was cloned and characterized for the
first time from
Phragmites australis. The full-length cDNA of
paepsps was 1308 bp encoding a polypeptide of 435
amino acids. The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants.
RT-PCR analysis of
paepsps expression indicated that the gene expressed in leaves, stems, and roots, with higher
expression in leaves. The expression of the
paepsps gene increased with glyphosate application. In addition, the
transgenic tobacco containing the
paepsps gene showed glyphosate resistance in comparison with control. The novel
paepsps is a good candidate gene in transgenic crops with glyphosate tolerance in the future.
Keywords
Cite This Article
WANG, Z., YUAN, Y. (2020). Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from
Phragmites australis.
BIOCELL, 44(4), 731–736.