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ARTICLE
Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton
MUHAMMAD HAMAYUN1,*, NASIR KHAN1, MUHAMMAD NAUMAN KHAN2, MUHAMMAD QADIR1, ANWAR HUSSAIN1, AMJAD IQBAL3, SUMERA AFZAL KHAN4, KHUSHNOOD UR REHMAN5, IN-JUNG LEE6,*
1 Plant-Microbe Interactions Lab, Department of Botany, Abdul Wali Khan University Mardan, KP, Pakistan
2 Department of Botany, Bacha Khan University Charsadda, Charsadda, 24420, Pakistan
3 Department of Food Science and Technology, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan
4 Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar, 25000, Pakistan
5 Department of Botany, Islamia College University, Peshawar, 25000, Pakistan
6 Department of Applied Biosciences, Kyungpook National University, Daegu, 41566, Korea
* Address correspondence to: In-Jung Lee, ; Muhammad Hamayun,
BIOCELL 2021, 45(2), 363-369. https://doi.org/10.32604/biocell.2021.013907
Received 26 August 2020; Accepted 22 November 2020; Issue published 19 February 2021
Abstract
Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the
host. Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as
well as stress conditions. The current study aimed to isolate endophytic bacteria from different parts of
Calotropis
procera, i.e., roots, stem and leaves of
Calotropis
procera (Ait.) W.T. Aiton. Plants were collected from the Lundkhwar,
district Mardan. A total of 12 bacterial strains, i.e., six from roots, three from the stem and three from the leaves were
isolated. The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and
easy response to the bioactive compounds present in the culture filtrate (CF) of the potent endophytic strains. The
rice plants were cultivated in pots containing 30 mL of 0.8% w/v water-agar medium. The pots were placed in a
growth chamber, operated at 28 ± 0.3°C for 14 h (day); and 25 ± 0.3°C for 10 h (night), at 70% relative-humidity.
Among the isolated strains, R1, S1, S3, L1, R5 and R6 showed visible growth promotion in rice plants. The
biochemical analysis revealed that the strains were able to produce indole acetic acid (IAA) and flavonoids in higher
quantities. Moreover, the strains also produced bioactive compounds that inhibited the growth of
Escherichia coli and
Aspergillus flavus using the well diffusion method. From the results, it was concluded that these strains can secrete
potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and,
therefore, can be used as bio-fertilizer and bio-control agents.
Keywords
Cite This Article
HAMAYUN, M., KHAN, N., KHAN, M. N., QADIR, M., HUSSAIN, A. et al. (2021). Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from
Calotropis procera (Ait.) W.T. Aiton.
BIOCELL, 45(2), 363–369.
Citations