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Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes

RONGXUE WEI1, CHUNCHUN HAN1,*, FENGJIANG YE1, SHOUHAI WEI2, FANG HE1, HEHE LIU1, LIANG LI1, HONGYONG XU1, SHENQIANG HU1, XIANYIN ZENG2
1 Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China
2 College of Life Science, Sichuan Agricultural University, Ya’an, 625014, China
* Corresponding Author: CHUNCHUN HAN. Email:

BIOCELL 2022, 46(1), 171-183. https://doi.org/10.32604/biocell.2022.015409

Received 17 December 2020; Accepted 30 December 2020; Issue published 28 September 2021

Abstract

In order to explore the role of forkhead box protein O1 (FoxO1) in the lipid metabolism and cell proliferation, goose primary hepatocytes were isolated and incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, and then transfected with FoxO1 interference plasmid. The related parameters of lipid metabolism and cell proliferation were measured. The results firstly showed that FoxO1 interference increased the intracellular TG and lipids concentration (P < 0.05); and increased the proliferative index (PI), cell DNA synthesis, protein expression of Cyclin D1 in goose primary hepatocytes (P < 0.05). Secondly, the co-treatment of insulin and FoxO1 interference increased the mRNA level and protein content of Cyclin D1 (P < 0.05); however, there was no significant difference between the insulin treatment and the co-treatment of insulin and miR-FoxO1 interference in the intracellular TG and lipids concentration and PI (P > 0.05). Lastly, the decrease of intracellular TG and lipids concentration and PI induced by NVP-BEZ235 was up-regulated by FoxO1 interference significantly (P < 0.05). In summary, FoxO1 could regulate the lipids metabolism and cell proliferation mediated by PI3K-Akt-mTOR signaling pathway in goose primary hepatocytes. Further investigations are required to highlight the potential role of FoxO1 in the lipid metabolism and cell proliferation mediated by insulin in goose primary hepatocyte.

Keywords

FoxO1; Lipid metabolism; Cell proliferation; PI3K-Akt-mTOR signal pathway; Goose primary hepatocytes

Cite This Article

WEI, R., HAN, C., YE, F., WEI, S., HE, F. et al. (2022). Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes. BIOCELL, 46(1), 171–183.



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