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Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition

HUOGEN LIU#, YUNDI SHI#, XIN WAN, YING LIU, HAILIN SHU, FENGMING HUANG, ZHENBIN GONG, LING GU*

Department of Critical Care Medicine, Mindong Hospital Affiliated to Fujian Medical University, Ningde, 355000, China

* Corresponding Author: LING GU. Email: email

(This article belongs to the Special Issue: Single Cell Technologies and Molecular Mechanisms of Diseases)

BIOCELL 2022, 46(12), 2671-2680. https://doi.org/10.32604/biocell.2022.018054

Abstract

Peroxiredoxin 1 (PRDX1) participates in tumor cell proliferation, apoptosis, migration, invasion, and the epithelial-to-mesenchymal transition (EMT). This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide (LPS) and transforming growth factor-beta 1 (TGF-β1). PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial (BEAS-2B) cells, reduced cell apoptosis (p < 0.01), and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase (MMP)2, MMP9, α-smooth muscle actin (α-SMA), N-cadherin, vimentin and twist proteins and inhibiting E-cadherin expression (p < 0.05). PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis (p < 0.05). Knockdown of PRDX1 inhibited cell proliferation, migration, EMT, and collagen synthesis (p < 0.01), reversed LPS-mediated inhibition of cell proliferation and migration, and significantly suppressed LPS-induced EMT and collagen synthesis (p < 0.01). The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.

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APA Style
LIU, H., SHI, Y., WAN, X., LIU, Y., SHU, H. et al. (2022). Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. BIOCELL, 46(12), 2671-2680. https://doi.org/10.32604/biocell.2022.018054
Vancouver Style
LIU H, SHI Y, WAN X, LIU Y, SHU H, HUANG F, et al. Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. BIOCELL . 2022;46(12):2671-2680 https://doi.org/10.32604/biocell.2022.018054
IEEE Style
H. LIU et al., "Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition," BIOCELL , vol. 46, no. 12, pp. 2671-2680. 2022. https://doi.org/10.32604/biocell.2022.018054



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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