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M1 macrophage-derived exosomes moderate the differentiation of bone marrow mesenchymal stem cells

TAILIN WU1,#, XIANG ZHOU2,#, CANHUA YE1, WENCAN LU1, HAITAO LIN1, YANZHE WEI1, ZEKAI KE1, ZHENGJI HUANG1, JIANZHOU LUO1, HUIREN TAO1, CHUNGUANG DUAN1,*
1 Shenzhen University General Hospital, Shenzhen, 518055, China
2 Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, 417000, China
* Corresponding Author: CHUNGUANG DUAN. Email:

BIOCELL 2022, 46(2), 495-503. https://doi.org/10.32604/biocell.2022.015214

Received 01 December 2020; Accepted 22 April 2021; Issue published 20 October 2021

Abstract

Differentiated macrophages have been proven to participate in the development of mesenchymal stem cells in different tissues. However, the regulatory processes remain obscure. Exosomes, which are key secretions of macrophages, have attracted increasing attention. Therefore, macrophage-derived exosomes may modulate the development of Bone marrow mesenchymal stem cells (BMMSCs). Different culture conditions were used to induce M1 polarization in THP1 cells. Subsequently, exosomes derived from unpolarized (M0) and polarized (M1) macrophages were isolated, BMMSCs were cultured with normal complete medium or inductive medium supplemented with M0 or M1 derived exosomes, and the osteogenic capacity of the BMMSCs was measured and analyzed. Finally, molecular mechanism associated with Akt and RUNX2 was investigated. Alizarin red staining and WB experiments showed that M1 macrophages could promote the osteogenic differentiation of BMMSCs better than M0 macrophages. Then, exosomes derived from M0 and M1 macrophages were successfully isolated and analyzed by electron microscopy and WB experiments. We concluded that media containing M1-derived exosomes promoted the osteogenic differentiation of BMMSCs better than media containing M0-derived exosomes. In addition, M1-derived exosomes could activate Akt and increase RUNX2 levels to promote osteogenesis. Our data demonstrated that exosomes derived from M1 macrophages induced osteogenesis by activating Akt and increasing RUNX2 level.

Keywords

M1 macrophages; Exosomes; Osteogenesis; RUNX2

Cite This Article

WU, T., ZHOU, X., YE, C., LU, W., LIN, H. et al. (2022). M1 macrophage-derived exosomes moderate the differentiation of bone marrow mesenchymal stem cells. BIOCELL, 46(2), 495–503.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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