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Tuning mesenchymal stem cell secretome therapeutic potential through mechanotransduction


1 Laboratório de Plasticidade e Diferenciação de Células da Crista Neural, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário–Trindade, CEP, Florianópolis, 88040-900, Brazil
2 Laboratório de Biologia Básica de Células-Tronco, Instituto Carlos Chagas, Fiocruz/PR, CEP, Curitiba, 81350-010, Brazil

* Address correspondence to: Giordano Wosgrau Calloni, giordano. email; Marco Augusto Stimamiglio, marco.stimamiglio@

(This article belongs to this Special Issue: Mesenchymal Stem Cells, Secretome and Biomaterials: Regenerative Medicine Application)

BIOCELL 2022, 46(6), 1375-1381.


Mesenchymal stem cells (MSCs) and their byproducts have been widely validated as potential therapeutic products for regenerative medicine. The therapeutic effects result mainly from the paracrine activity of MSCs, which consists of the secretion of bioactive molecules, whether dispersed in medium conditioned by cell culture or encapsulated in extracellular vesicles. The composition of the MSC secretome, which represents the set of these secreted cellular products, is crucial for the performance of the desired therapeutic functions. Different cell culture strategies have been employed to adjust the secretome composition of MSCs to obtain the best therapeutic responses for different clinical contexts. However, the manipulation of culture conditions has focused mainly on the use of different biochemical elements for the preconditioning of MSCs and less on the physical conditions of the cell culture environment. Herein, we offer our point of view regarding the importance of the physical properties of cell culture substrates and their mechanotransduction responses in preconditioning the MSCs secretome. We highlight the relevance of studying mechanotransduction events associating cell morphology and the modulation of gene expression to customize and expand the use of MSCs secretomes.


Cite This Article

CALLONI, G. W., STIMAMIGLIO, M. A. (2022). Tuning mesenchymal stem cell secretome therapeutic potential through mechanotransduction. BIOCELL, 46(6), 1375–1381.


cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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