Open Access
ARTICLE
Overexpression of a sugarcane ScCaM gene negatively regulates salinity and drought stress responses in transgenic Arabidopsis thaliana
JINXIAN LIU1, JINGFANG FENG2, CHANG ZHANG2, YONGJUAN REN2, WEIHUA SU2, GUANGHENG WU1, XIANYU FU1, NING HUANG3, YOUXIONG QUE2, HUI LING3,*, JUN LUO2,*
1 College of Tea and Food Science; Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, Wuyi University, Nanping, 354300, China
2 Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture; Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops,
Ministry of Education, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China
3 College of Agriculture, Yulin Normal University, Yulin, 537000, China
* Corresponding Authors: Hui Ling, ; Jun Luo,
(This article belongs to this Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)
BIOCELL 2023, 47(1), 215-225. https://doi.org/10.32604/biocell.2022.022477
Received 11 March 2022; Accepted 20 May 2022; Issue published 26 September 2022
Abstract
Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study,
the effects of a sugarcane
ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in
transgenic
Arabidopsis thaliana and
Escherichia coli cells were evaluated. The results demonstrated a significant
negative role of
ScCaM in the drought and salt stress tolerance of transgenic lines of
A. thaliana, as indicated by the
phenotypes. In addition, the expression of
AtP5CS and
AtRD29A, two genes tightly related to stress resistance, was
significantly lower in the overexpression lines than in the wild type. The growth of
E. coli BL21 cells expressing
ScCaM showed weaker tolerance under mannitol and NaCl stress. Taken together, this study revealed that the
ScCaM
gene plays a negative regulatory role in both mannitol and NaCl stresses, and it possibly exerts protective mechanisms
common in both prokaryotes and eukaryotes under stress conditions.
Keywords
Cite This Article
LIU, J., FENG, J., ZHANG, C., REN, Y., SU, W. et al. (2023). Overexpression of a sugarcane
ScCaM gene negatively regulates salinity and drought stress responses in transgenic
Arabidopsis thaliana.
BIOCELL, 47(1), 215–225.