Open Access iconOpen Access

ARTICLE

crossmark

Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of Arachis hypogaea (Fabales: Fabaceae) against Meloidogyne incognita (Tylenchida: Heteroderidae)

XUEJIN YANG1,2,#, YUANYUAN ZHOU1,#, XINYI PENG1, XIAOHONG FU1, JIANQING MA1, JIANFENG LIU1,*, DANDAN CAO1,2,*

1 College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China
2 Hebei Innovation Center for Bioengineering and Biotechnology, Hebei University, Baoding, 071002, China

* Corresponding Authors: Jianfeng Liu, email; Dandan Cao, email
# These authors contributed equally to this work

(This article belongs to the Special Issue: Plant Cell Physiology and Stress Biology)

BIOCELL 2023, 47(9), 2101-2113. https://doi.org/10.32604/biocell.2023.029581

Abstract

Background: Plant root-knot nematode (RKN) disease is a serious threat to agricultural production across the world. Meloidogyne incognita is the most prominent pathogen to the vegetables and cash crops cultivated. Arachis hypogaea can effectively inhibit M. incognita, but the underlying defense mechanism is still unclear. Methods: In our study, the chemotaxis and infestation of the second-stage juveniles (J2s) of M. incognita to A. hypogaea root tips were observed by the Pluronic F-127 system and stained with sodium hypochlorite acid fuchsin, respectively. The transcriptome data of A. hypogaea roots with non-infected or infected by J2s were analyzed. Results: The J2s could approach and infect inside of A. hypogaea root tips, and the chemotactic migration rate and infestation rate were 20.72% and 22.50%, respectively. Differential gene expression and pathway enrichment analyses revealed ubiquinone and other terpenoid-quinone biosynthesis pathway, plant hormone signal transduction pathway, and phenylpropanoid biosynthesis pathway in A. hypogaea roots responded to the infestation of M. incognita. Furthermore, the AhHPT gene, encoding homogentisate phytyltransferase, was considered to be an ideal candidate gene due to its higher expression based on the transcriptome data and quantitative real-time PCR analysis. Conclusion: Therefore, the key gene AhHPT might be involved in the A. hypogaea against M. incognita. These findings lay a foundation for revealing the molecular mechanism of A. hypogaea resistance to M. incognita and also provide a prerequisite for further gene function verification, aiming at RKN-resistant molecular breeding.

Keywords


Cite This Article

APA Style
YANG, X., ZHOU, Y., PENG, X., FU, X., MA, J. et al. (2023). Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of arachis hypogaea (fabales: fabaceae) against meloidogyne incognita (tylenchida: heteroderidae). BIOCELL, 47(9), 2101-2113. https://doi.org/10.32604/biocell.2023.029581
Vancouver Style
YANG X, ZHOU Y, PENG X, FU X, MA J, LIU J, et al. Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of arachis hypogaea (fabales: fabaceae) against meloidogyne incognita (tylenchida: heteroderidae). BIOCELL . 2023;47(9):2101-2113 https://doi.org/10.32604/biocell.2023.029581
IEEE Style
X. YANG et al., "Comparative transcriptome analysis provides insights into the molecular mechanism of the anti-nematode role of Arachis hypogaea (Fabales: Fabaceae) against Meloidogyne incognita (Tylenchida: Heteroderidae)," BIOCELL , vol. 47, no. 9, pp. 2101-2113. 2023. https://doi.org/10.32604/biocell.2023.029581



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 620

    View

  • 300

    Download

  • 0

    Like

Share Link