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A Rapid Parameter of Enzyme-Treated Cellulosic Material Revealed by Reducing Sugar Release

Verônica Távilla Ferreira Silva, Adriane Maria Ferreira Milagres*

Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, 12602-810, Brazil

* Corresponding Author: Adriane Maria Ferreira Milagres. Email: email

(This article belongs to the Special Issue: Renewable Materials and Advanced Technologies for Sustainability)

Journal of Renewable Materials 2024, 12(3), 539-551. https://doi.org/10.32604/jrm.2023.045726

Abstract

This study was conducted to evaluate the effectiveness of enzymes in purifying and reducing the degree of polymerization of cellulose for the production of dissolving pulp. Our goal was to determine the contributions of xylanase (X) and endoglucanase (EG) in the treatment of pulp, specifically by quantifying the formation of soluble and insoluble reducing sugars using the dinitrosalycilic acid (DNS) test. Predominantly, the release of soluble reducing sugars (RSSol) was enhanced after xylanase treatment, while endoglucanase (EG) treatment led to changes in insoluble reducing sugars (RSIns). The maximum synergism was observed for RSIns when a high ratio of endoglucanase to xylanase (320EG:5X/g pulp) was used. The relative contribution of endoglucanase to RSins was determined to be 15.6% of the total reducing sugar. The viscosity of pulps treated with xylanase decreased only by 7%, whereas endoglucanase treatment significantly reduced viscosity by 45%. Modifications in the particle size were observed after pulp treatment with the combination of endoglucanase and xylanase. In summary, the DNS test is a rapid and effective method for evaluating the efficiency of enzyme treatments on pulps. The measurement of RSIns correlates with changes in pulp viscosity to different extents, providing valuable insights into the effectiveness of enzyme treatments.

Graphical Abstract

A Rapid Parameter of Enzyme-Treated Cellulosic Material Revealed by Reducing Sugar Release

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Cite This Article

APA Style
Silva, V.T.F., Milagres, A.M.F. (2024). A rapid parameter of enzyme-treated cellulosic material revealed by reducing sugar release. Journal of Renewable Materials, 12(3), 539-551. https://doi.org/10.32604/jrm.2023.045726
Vancouver Style
Silva VTF, Milagres AMF. A rapid parameter of enzyme-treated cellulosic material revealed by reducing sugar release. J Renew Mater. 2024;12(3):539-551 https://doi.org/10.32604/jrm.2023.045726
IEEE Style
V.T.F. Silva and A.M.F. Milagres, "A Rapid Parameter of Enzyme-Treated Cellulosic Material Revealed by Reducing Sugar Release," J. Renew. Mater., vol. 12, no. 3, pp. 539-551. 2024. https://doi.org/10.32604/jrm.2023.045726



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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