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Induction of Multidrug Resistance of Acute Myeloid Leukemia Cells by Cocultured Stromal Cells via Upregulation of the PI3K/Akt Signaling Pathway

Ping Chen*, Qing Jin*, Qiang Fu*, Peidong You*, Xi Jiang*, Qin Yuan*, Huifang Huang

* Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fujian, P.R. China
† Central Laboratory, Fujian Medical University Union Hospital, Fujian, P.R. China

Oncology Research 2016, 24(4), 215-223. https://doi.org/10.3727/096504016X14634208143021

Abstract

This study aimed to investigate the role of the PI3K/Akt signaling pathway in multidrug resistance of acute myeloid leukemia (AML) cells induced by cocultured stromal cells. Human AML cell lines HL-60 and U937 were adhesion cocultured with human bone marrow stromal cell line HS-5 cells. Such coculturing induced HL-60 and U937 cells resistant to chemotherapeutic drugs including daunorubicin (DNR), homoharringtonine (HHT), and cytosine arabinoside (Ara-C). The coculturing-induced resistance of AML cells to DNR, HHT, and Ara-C can be partially reversed by inhibition of the PI3K/Akt signaling pathway. Clinically, AML patients with a low level of PTEN and a high level of CCND1 had high relapse rates within 1 year, and newly diagnosed AML patients with extramedullary infiltration had a low level of PTEN. This study confirms the involvement of the PI3K/Akt signaling pathway in multidrug resistance in AML cells induced by stroma and suggests that the expression of PTEN and CCND1 may be a prognostic indicator for AML.

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APA Style
Chen, P., Jin, Q., Fu, Q., You, P., Jiang, X. et al. (2016). Induction of multidrug resistance of acute myeloid leukemia cells by cocultured stromal cells via upregulation of the pi3k/akt signaling pathway. Oncology Research, 24(4), 215-223. https://doi.org/10.3727/096504016X14634208143021
Vancouver Style
Chen P, Jin Q, Fu Q, You P, Jiang X, Yuan Q, et al. Induction of multidrug resistance of acute myeloid leukemia cells by cocultured stromal cells via upregulation of the pi3k/akt signaling pathway. Oncol Res. 2016;24(4):215-223 https://doi.org/10.3727/096504016X14634208143021
IEEE Style
P. Chen et al., "Induction of Multidrug Resistance of Acute Myeloid Leukemia Cells by Cocultured Stromal Cells via Upregulation of the PI3K/Akt Signaling Pathway," Oncol. Res., vol. 24, no. 4, pp. 215-223. 2016. https://doi.org/10.3727/096504016X14634208143021



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