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MicroRNA-133a Inhibits Proliferation of Gastric Cancer Cells by Downregulating ERBB2 Expression

Chang Li*, Xiaoping Li, Shuohui Gao*, Chang Li, Lianjun Ma§

* Department of Gastrointestinal Internal Medicine, China–Japan Union Hospital of Jilin University, Changchun, P.R. China
† Department of Pediatrics, The First Hospital of Jilin University, Changchun, P.R. China
‡ Department of Cadre’s Ward, China–Japan Union Hospital of Jilin University, Changchun, P.R. China
§ Endoscopy Center, China–Japan Union Hospital of Jilin University, Changchun, P.R. China

Oncology Research 2017, 25(7), 1169-1176. https://doi.org/10.3727/096504017X14847395834985

Abstract

Gastric cancer is the fourth most common type of cancer and the second highest leading cause of cancer-related deaths worldwide. It has already been established that miR-133a is involved in gastric cancer. In this study, we investigated the molecular mechanisms by which miR-133a inhibits the proliferation of gastric cancer cells. We analyzed the proliferative capacity of human gastric cancer cells SNU-1 using an MTT assay. Cell apoptosis was determined using flow cytometry. The expression levels of ERBB2, p-ERK1/2, and p-AKT in SNU-1 cells were determined using Western blot analysis. To confirm that ERBB2 is a direct target of miR-133a, a luciferase reporter assay was performed. Results showed that miR-133a overexpression inhibited SNU-1 cell proliferation and increased apoptosis. ERBB2 was a direct target of miR-133a, and it was negatively regulated by miR-133a. Interestingly, ERBB2 silencing has a similar impact to miR-133a overexpression, in that it significantly induced apoptosis and inhibited ERK and AKT activation. Our study showed that miR-133a inhibits the proliferation of gastric cancer cells by downregulating the expression of ERBB2 and its downstream signaling molecules p-ERK1/2 and p-AKT. Therefore, miR-133a might be used as a therapeutic target for treating gastric cancer.

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APA Style
Li, C., Li, X., Gao, S., Li, C., Ma, L. (2017). Microrna-133a inhibits proliferation of gastric cancer cells by downregulating ERBB2 expression. Oncology Research, 25(7), 1169-1176. https://doi.org/10.3727/096504017X14847395834985
Vancouver Style
Li C, Li X, Gao S, Li C, Ma L. Microrna-133a inhibits proliferation of gastric cancer cells by downregulating ERBB2 expression. Oncol Res. 2017;25(7):1169-1176 https://doi.org/10.3727/096504017X14847395834985
IEEE Style
C. Li, X. Li, S. Gao, C. Li, and L. Ma "MicroRNA-133a Inhibits Proliferation of Gastric Cancer Cells by Downregulating ERBB2 Expression," Oncol. Res., vol. 25, no. 7, pp. 1169-1176. 2017. https://doi.org/10.3727/096504017X14847395834985



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