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MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8

Jin Wang*, Wenquan Pang*, Zhenbai Zuo*, Wenyan Zhang*, Weidong He

* Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao, P.R. China
† Department of Nutriology, The Affiliated Hospital of Qingdao University, Qingdao, P.R. China

Oncology Research 2017, 25(8), 1297-1304. https://doi.org/10.3727/096504017X14873430389189

14 June 2024 Editors Note:

Readers are alerted that concerns were raised about the integrity of some of the data presented in the article. Further editorial action will be taken once this matter has been resolved.

Abstract

Spinal osteosarcoma (OS) is a malignant tumor that has a poor outcome. MicroRNA-520b (miR-520b) acts as a cancer suppressor in various types of cancer. Because of the limited amount of literature on OS, we aimed to identify the role of miR-520b in OS. The miR-520b level in clinical spinal OS tissues and adjacent nontumor tissues as well as in cell lines was assessed. The effect of miR-520b on cell proliferation, migration, invasion, and frizzled-8 (FZD8) degradation were all evaluated. Alterations of key proteins involved in the Wnt/b-catenin pathway were assessed by Western blot analysis. In the present study, miR-520b was downregulated in human spinal OS tissues and OS cell lines (p<0.01 or p<0.001). Overexpression of miR-520b inhibited cell proliferation (p<0.01 or p<0.001), migration (p<0.01), and invasion (p<0.01). FZD8 expression was negatively regulated by infection with a lentivirus vector carrying an miR-520b precursor in dose- and time-dependent manners. In OS tissues, miR-520b was inversely correlated with FZD8 expression. FZD8 was upregulated in human spinal OS tissues and cell lines. Finally, miR-520b inactivated the Wnt/β-catenin pathway through downregulation of FZD8. miR-520b inhibited cell proliferation, migration, and invasion through inactivating the Wnt/β-catenin pathway by downregulation of FZD8, providing a novel therapeutic target for spinal OS.

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Cite This Article

APA Style
Wang, J., Pang, W., Zuo, Z., Zhang, W., He, W. (2017). Microrna-520b suppresses proliferation, migration, and invasion of spinal osteosarcoma cells via downregulation of frizzled-8. Oncology Research, 25(8), 1297-1304. https://doi.org/10.3727/096504017X14873430389189
Vancouver Style
Wang J, Pang W, Zuo Z, Zhang W, He W. Microrna-520b suppresses proliferation, migration, and invasion of spinal osteosarcoma cells via downregulation of frizzled-8. Oncol Res. 2017;25(8):1297-1304 https://doi.org/10.3727/096504017X14873430389189
IEEE Style
J. Wang, W. Pang, Z. Zuo, W. Zhang, and W. He "MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8," Oncol. Res., vol. 25, no. 8, pp. 1297-1304. 2017. https://doi.org/10.3727/096504017X14873430389189



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