Open Access

ARTICLE

Silencing of peroxiredoxin 2 suppresses proliferation and Wnt/β-catenin pathway, and induces senescence in hepatocellular carcinoma

XUEGANG YANG^1,#, XIANHONG XIANG^2,3,#, GUOHUI XU¹, SHI ZHOU³, TIANZHI AN^3,4,*, ZHI HUANG^3,4,*

1 Department of Interventional Radiology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Radiation Oncology Key Laboratory of Sichuan Province, Chengdu, 610041, China
2 Department of Interventional Radiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China
3 Department of Interventional Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China
4 School of Basic Medical Science, Guizhou Medical University, Guiyang, 550002, China

* Corresponding Authors: TIANZHI AN. Email: ; ZHI HUANG. Email:
# These authors contributed equally to this work

(This article belongs to the Special Issue: Role of Reactive Oxygen Species and DNA Damage in Tumor Immunological Responses)

Oncology Research 2024, 32(1), 213-226. https://doi.org/10.32604/or.2023.030768

Received 21 April 2023; Accepted 03 August 2023; Issue published 15 November 2023

Abstract

Hepatocellular carcinoma (HCC), a common malignancy worldwide, still lacks effective clinical treatment. The study aimed to investigate the oncogenes that affect the progression of HCC and their possible mechanisms. In our study, we initially confirmed a higher level of PRDX2 in the bile of HCC patients compared to those with choledocholithiasis by 2-DE, LC-MS, and ELISA. Subsequently, we demonstrated the high expression of peroxiredoxin 2 (PRDX2) in HCC based on the TCGA database and clinical sample analysis. Furthermore, PRDX2 overexpression enhanced the viability of HCC cells. And PRDX2 silencing induced senescence of HCC cells. In vivo, knockdown of PRDX2 significantly reduced the weight of xenograft tumors. PRDX2 also was found to activate the Wnt/β-catenin pathway by inducing β-catenin nuclear translocation. Consequently, we proved that silencing PRDX2 could inhibit proliferation and Wnt/β-catenin pathway while promoting senescence in HCC cells.

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Keywords

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