Open Access iconOpen Access

ARTICLE

crossmark

Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities

GANG WANG#, MINGPING QIAN#, WEI JIAN, JUHANG CHU, YIXIANG HUANG*

Department of General Surgery, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China

* Corresponding Author: Yixiang Huang, email
# These authors contributed equally to this work

Oncology Research 2023, 31(1), 63-70. https://doi.org/10.32604/or.2022.028168

Abstract

Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction, has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069 was highly expressed in breast cancer tissues and cells. The expression of has_circ_0000069 was associated with the five-year overall survival of patients. After silencing has_circ_0000069 in breast cancer cells, its expression reduced, and the ability of cell proliferation, migration, and invasion decreased. MiR-432 was verified as a targeting miRNA of has_circ_0000069. Has_circ_0000069 expression increased in breast cancer and was negatively related to patient’s prognosis. Has_circ_0000069 may facilitate breast cancer tumor progression by sponging miR-432. These findings revealed that has_circ_0000069 may be a biomarker for predicting prognosis and a therapeutic target for treating patients with breast cancer.

Keywords


Cite This Article

APA Style
WANG, G., QIAN, M., JIAN, W., CHU, J., HUANG, Y. (2023). Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities. Oncology Research, 31(1), 63-70. https://doi.org/10.32604/or.2022.028168
Vancouver Style
WANG G, QIAN M, JIAN W, CHU J, HUANG Y. Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities. Oncol Res. 2023;31(1):63-70 https://doi.org/10.32604/or.2022.028168
IEEE Style
G. WANG, M. QIAN, W. JIAN, J. CHU, and Y. HUANG "Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities," Oncol. Res., vol. 31, no. 1, pp. 63-70. 2023. https://doi.org/10.32604/or.2022.028168



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1027

    View

  • 527

    Download

  • 0

    Like

Share Link