Table of Content

Open Access iconOpen Access

ARTICLE

miR-136 Inhibits Malignant Progression of Hepatocellular Carcinoma Cells by Targeting Cyclooxygenase 2

Haiyan Jia*, Hong Wang, Yanfen Yao*, Chunlei Wang, Pibao Li*

* Department of Intensive Care Unit, Shandong Provincial Third Hospital, Tianqiao District, Jinan, Shandong, P.R. China
† Department of General Surgery, Shandong Provincial Third Hospital, Tianqiao District, Jinan, Shandong, P.R. China
‡ Thyroid Disease Prevention and Control Center, Shandong Provincial Institute of Endemic Disease Control, Lixia District, Jinan, Shandong, P.R. China

Oncology Research 2018, 26(6), 967-976. https://doi.org/10.3727/096504018X15148192843443

Abstract

MicroRNAs (miRNAs) play a vital role in regulating tumor progression. Dysregulated miR-136 expression was linked to the development of various human cancers. In the present study, we investigated the expression and relationship of miR-136 and COX2 in hepatocellular carcinoma (HCC) using relevant experiments, involving CCK-8, Transwell assay, and luciferase reporter assay. We demonstrated that miR-136 expression is obviously decreased in HCC tissues and cells, and negatively correlated with the expression of COX2 mRNA. In vitro assay revealed that overexpression of miR-136 significantly changed the expression of proliferation- and metastasis-related proteins and inhibited the proliferation, migration, and invasion of HepG2 and Hep3B cells. Dual-luciferase reporter assay validated that the 3¢-untranslated region (3¢-UTR) of COX2 is a direct target of miR-136. Furthermore, COX2 siRNA partially enhanced the miR-136 overexpression-induced inhibitory effects. In conclusion, miR-136 was vital in the regulation of HCC cell proliferation and metastasis by targeting COX2. Thus, our findings provided novel evidence that miR-136 might be recommended as a potential target for the diagnosis and treatment of HCC patients.

Keywords


Cite This Article

APA Style
Jia, H., Wang, H., Yao, Y., Wang, C., Li, P. (2018). Mir-136 inhibits malignant progression of hepatocellular carcinoma cells by targeting cyclooxygenase 2. Oncology Research, 26(6), 967-976. https://doi.org/10.3727/096504018X15148192843443
Vancouver Style
Jia H, Wang H, Yao Y, Wang C, Li P. Mir-136 inhibits malignant progression of hepatocellular carcinoma cells by targeting cyclooxygenase 2. Oncol Res. 2018;26(6):967-976 https://doi.org/10.3727/096504018X15148192843443
IEEE Style
H. Jia, H. Wang, Y. Yao, C. Wang, and P. Li "miR-136 Inhibits Malignant Progression of Hepatocellular Carcinoma Cells by Targeting Cyclooxygenase 2," Oncol. Res., vol. 26, no. 6, pp. 967-976. 2018. https://doi.org/10.3727/096504018X15148192843443



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 55

    View

  • 48

    Download

  • 0

    Like

Share Link