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miR-522-3p Promotes Tumorigenesis in Human Colorectal Cancer via Targeting Bloom Syndrome Protein

Feng Shuai*, Bo Wang, Shuxiao Dong

* Department of Gastroenterology, Eastern District of Linyi People’s Hospital, Linyi, Shandong, P.R. China
† Department of Pediatrics, Chinese Medicine Hospital in Linyi City, Linyi, Shandong, P.R. China
‡ Department of Gastrointestinal Surgery, Linyi People’s Hospital, Linyi, Shandong, P.R. China

Oncology Research 2018, 26(7), 1113-1121. https://doi.org/10.3727/096504018X15166199939341

Abstract

miR-522-3p is known to degrade bloom syndrome protein (BLM) and enhance expression of other proto-oncogenes, leading to tumorigenesis. This study aimed to investigate the molecular mechanisms of miR-522-3p in human colorectal cancer (CRC) cells. Expressions of miR-522-3p in CRC and adjacent tissues, as well as in normal human colon epithelial cell line (FHC) and five CRC cell lines, were detected. Human CRC cell lines, HCT-116 and HT29, were transfected with miR-522-3p mimic, inhibitor, or scrambled controls. Then cell viability, apoptosis, cell cycle progression, and the expressions of c-myc, cyclin E, CDK2, and BLM were assessed. It was found that miR-522-3p was highly expressed in CRC tissues when compared to adjacent nontumor tissues and was highly expressed in CRC cell lines when compared to FHC cells. miR-522-3p overexpression promoted cell viability, reduced apoptotic cell rate, arrested more cells in the S phase, and upregulated c-myc, cyclin E, and CDK2 expression. BLM was a target gene of miR-522-3p, and miR-522-3p suppression did not exert antiproliferative and proapoptotic activities when BLM was silenced. These findings demonstrate that miR-522-3p upregulation negatively regulates the expression of BLM, with upregulation of c-myc, CDK2, and cyclin E, and thereby promoting the proliferation of human CRC cells.

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APA Style
Shuai, F., Wang, B., Dong, S. (2018). Mir-522-3p promotes tumorigenesis in human colorectal cancer via targeting bloom syndrome protein. Oncology Research, 26(7), 1113-1121. https://doi.org/10.3727/096504018X15166199939341
Vancouver Style
Shuai F, Wang B, Dong S. Mir-522-3p promotes tumorigenesis in human colorectal cancer via targeting bloom syndrome protein. Oncol Res. 2018;26(7):1113-1121 https://doi.org/10.3727/096504018X15166199939341
IEEE Style
F. Shuai, B. Wang, and S. Dong "miR-522-3p Promotes Tumorigenesis in Human Colorectal Cancer via Targeting Bloom Syndrome Protein," Oncol. Res., vol. 26, no. 7, pp. 1113-1121. 2018. https://doi.org/10.3727/096504018X15166199939341



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