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miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1

Jie Shen*, Jianhua Zhang, Minhui Xiao*, Junfeng Yang*, Ningnan Zhang*

* Department of Urology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, P.R. China
† Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, P.R. China

Oncology Research 2018, 26(8), 1155-1165.


miR-203 is an epigenetically silenced tumor-suppressive microRNA in tumors. This study was designed to investigate the effects of miR-203 on the proliferation, migration, invasion, and apoptosis of bladder cancer (BCa) cells. The expression levels of miR-203 in BCa tissues, normal adjacent tissues, and BCa cell lines were detected. BCa cells were transfected with miR-203 mimic and inhibitor to investigate the effect of miR-203 on cell functions and the epithelial–mesenchymal transition (EMT). Cotransfection with miR-203 inhibitor and shRNA of the predicted target gene Twist1 (si-Twist1) was performed to investigate the target relationship of miR-203 and Twist1. The effects of knockdown of Twist1 on cell functions were also investigated. The expression of miR-203 was significantly reduced in BCa tissues and cells, in comparison with the control. miR-203 mimic significantly reduced cell viability, invasion, migration, and EMT, and enhanced cell apoptosis. On the contrary, miR-203 inhibitor showed the opposite results. However, the administration of si-Twist1 cancelled the effect of miR-203 inhibitor on cell proliferation, apoptosis, invasion, and migration. These demonstrated that miR-203 may function as a tumor-suppressive microRNA in BCa by negatively targeting Twist1. Both Twist1 and miR-203 might be explored as potential targets for studying the mechanism related to BCa pathogenesis and therapy.


Cite This Article

APA Style
Shen, J., Zhang, J., Xiao, M., Yang, J., Zhang, N. (2018). Mir-203 suppresses bladder cancer cell growth and targets twist1. Oncology Research, 26(8), 1155-1165.
Vancouver Style
Shen J, Zhang J, Xiao M, Yang J, Zhang N. Mir-203 suppresses bladder cancer cell growth and targets twist1. Oncol Res. 2018;26(8):1155-1165
IEEE Style
J. Shen, J. Zhang, M. Xiao, J. Yang, and N. Zhang "miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1," Oncol. Res., vol. 26, no. 8, pp. 1155-1165. 2018.

cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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