Table of Content

Open Access iconOpen Access

ARTICLE

MicroRNA-331 Inhibits Proliferation and Invasion of Melanoma Cells by Targeting Astrocyte-Elevated Gene-1

Li Chen, Guozhang Ma, Xiaohui Cao, Xiaoxia An, Xiguang Liu

Department of Dermatology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, P.R. China

Oncology Research 2018, 26(9), 1429-1437. https://doi.org/10.3727/096504018X15186047251584

Abstract

Melanoma is characterized by aggressive invasion, early metastasis, and resistance to existing chemotherapeutic agents. Accumulated studies have reported that microRNA (miRNA) is a potentially robust molecular tool for developing future therapeutic technologies. Therefore, examining the expression patterns, biological roles, and associated mechanisms of cancer-related miRNAs in melanoma is essential for developing novel therapeutic targets for patients with this disease. In this study, miRNA-331 (miR-331) was underexpressed in melanoma tissues and cell lines. Functional assays revealed that the enforced expression of miR-331 inhibited cell proliferation and invasion. In addition, astrocyte-elevated gene-1 (AEG-1) was identified as a novel target of miR- 331 through bioinformatics analysis, reverse transcription quantitative polymerase chain reaction analysis, Western blot analysis, dual-luciferase reporter assay, and Spearman’s correlation analysis. Furthermore, reintroduction of AEG-1 partially abrogated the inhibitory effects of miR-331 overexpression on the proliferation and invasion of melanoma cells. Moreover, miR-331 suppressed the activation of the PTEN/AKT signaling pathway in melanoma by inhibiting AEG-1. In short, miR-331 may play tumor-suppressive roles in melanoma by directly targeting AEG-1 and regulating the PTEN/AKT signaling pathway, suggesting that miR-331 could be investigated as a therapeutic strategy for patients with this malignancy.

Keywords


Cite This Article

APA Style
Chen, L., Ma, G., Cao, X., An, X., Liu, X. (2018). Microrna-331 inhibits proliferation and invasion of melanoma cells by targeting astrocyte-elevated gene-1. Oncology Research, 26(9), 1429-1437. https://doi.org/10.3727/096504018X15186047251584
Vancouver Style
Chen L, Ma G, Cao X, An X, Liu X. Microrna-331 inhibits proliferation and invasion of melanoma cells by targeting astrocyte-elevated gene-1. Oncol Res. 2018;26(9):1429-1437 https://doi.org/10.3727/096504018X15186047251584
IEEE Style
L. Chen, G. Ma, X. Cao, X. An, and X. Liu "MicroRNA-331 Inhibits Proliferation and Invasion of Melanoma Cells by Targeting Astrocyte-Elevated Gene-1," Oncol. Res., vol. 26, no. 9, pp. 1429-1437. 2018. https://doi.org/10.3727/096504018X15186047251584



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 40

    View

  • 32

    Download

  • 0

    Like

Share Link