Open Access
ARTICLE
lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 (GLI2)
Hongliang Yang*1,
Lei Yan†1,
Kai Sun‡, Xiaodong Sun†, Xudong Zhang,§ Kerui Cai†, Tiejun Song*
* Department of Clinical Laboratory, The Second Affiliated Hospital of Mudanjiang Medical University,
Mudanjiang, Heilongjiang, P.R. China
† Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, P.R. China
‡ Department of Biology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, P.R. China
§ Department of Physiology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, P.R. China
Oncology Research 2019, 27(3), 359-369. https://doi.org/10.3727/096504018X15220594629967
Abstract
This study aimed to explore the effects of lncRNA BCAR4 on the viability and aggressiveness of non-small cell
lung cancer (NSCLC) cells. qRT-PCR was used to determine the expression of BCAR4 and
GLI2 downstream
genes in NSCLC tissues and cell lines. Chromatin isolation by RNA purification (CHIRP) and Western blot
were employed to measure the expression of the
GLI2 downstream proteins. Ki-67 expression in nude mice
tumors was tested by immunohistochemistry. MTT assay, wound healing assay, and Transwell assay were used
to assess NSCLC cell viability and aggressiveness, respectively. Tumor xenograft was conducted to determine
the effects of BCAR4 and
GLI2 on NSCLC tumorigenesis in vivo. The expression of BCAR4 in NSCLC tissues and cells was significantly higher than the normal level. The overexpression of BCAR4 promoted NSCLC
cell viability, migration, and invasion. The suppression of BCAR4 and
GLI2 showed the opposite effects.
The overexpression of BCAR4 led to an increase in the expression of
GLI2 downstream proteins, while the
suppression of BCAR4 and
GLI2 reduced their expression. In a tumor xenograft assay, the tumors in mice of
the BCAR4 group showed the biggest volume, while those in mice of the si-GLI2 group showed the smallest
volume. Ki-67 showed much higher levels in the BCAR4 overexpression group but much lower levels in the
si-GLI2 group. In summary, the cooperative mechanism of lncRNA BCAR4 and
GLI2 might provide a new
opportunity for treating NSCLC.
Keywords
Cite This Article
Yang, H., Yan,
. L., Sun,
. K., Sun, X., Zhang,§, X. et al. (2019). lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 (
GLI2).
Oncology Research, 27(3), 359–369.