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Inhibition of Beclin-1-Mediated Autophagy by MicroRNA-17-5p Enhanced the Radiosensitivity of Glioma Cells

Weichen Hou*, Lei Song, Yang Zhao*, Qun Liu*, Shuyan Zhang

* Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin Province, P.R. China
† Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun, Jilin Province, P.R. China
‡ Department of Neurotrauma, The First Hospital of Jilin University, Changchun, Jilin Province, P.R. China

Oncology Research 2020, 28(7-8), 815-818. https://doi.org/10.3727/096504021X16240202939988

Abstract

The role of miRNAs in the radiosensitivity of glioma cells and the underlying mechanism is still far from clear. In the present study, we detected six downregulated and seven upregulated miRNAs in the serum after radiotherapy compared with paired serum samples before radiotherapy via miRNA panel PCR. Among these, miR-17-5p was highly reduced (fold change = −4.21). Further, we validated the levels of miR-17-5p in all serum samples with qRT-PCR. In addition, statistical analysis suggested that a reduced miR-17-5P level was positively associated with advanced clinical stage of glioma, incidence of relapse, and tumor differentiation. Moreover, we provided evidence that irradiation markedly activated autophagy and decreased miR-17-5p in the glioma cell line. Further, we demonstrated that irradiation-induced autophagy activation was mediated by beclin-1, and downregulation of beclin-1 via siRNA significantly abolished the irradiation-activated autophagy. Interestingly, we demonstrated that miR-17-5p could directly target beclin-1 via luciferase gene reporter assay. Exotic expression of miRNA-17-5p decreased autophagy activity in vitro. In nude mice, miRNA-17-5p upregulation sensitized the xenograft tumor to irradiation and suppressed irradiation-induced autophagy. Finally, pharmacal inhibition of autophagy markedly enhanced the cytotoxicity of irradiation in RR-U87 cells.

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APA Style
Hou, W., Song, L., Zhao, Y., Liu, Q., Zhang, S. (2020). Inhibition of beclin-1-mediated autophagy by microrna-17-5p enhanced the radiosensitivity of glioma cells. Oncology Research, 28(7-8), 815-818. https://doi.org/10.3727/096504021X16240202939988
Vancouver Style
Hou W, Song L, Zhao Y, Liu Q, Zhang S. Inhibition of beclin-1-mediated autophagy by microrna-17-5p enhanced the radiosensitivity of glioma cells. Oncol Res. 2020;28(7-8):815-818 https://doi.org/10.3727/096504021X16240202939988
IEEE Style
W. Hou, L. Song, Y. Zhao, Q. Liu, and S. Zhang "Inhibition of Beclin-1-Mediated Autophagy by MicroRNA-17-5p Enhanced the Radiosensitivity of Glioma Cells," Oncol. Res., vol. 28, no. 7-8, pp. 815-818. 2020. https://doi.org/10.3727/096504021X16240202939988



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