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LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral squamous carcinoma

FAYU SHAN1, LANLAN LIANG1, CHONG FENG1, HONGBAO XU1, ZIROU WANG1, WEILI LIU1, LINGLING PU1, ZHAOLI CHEN1, GANG CHEN2,*, XINXING WANG1,*

1 Department of Environmental Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300070, China
2 School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300050, China

* Corresponding Authors: GANG CHEN. Email: email; XINXING WANG. Email: email

Oncology Research 2023, 31(4), 481-493. https://doi.org/10.32604/or.2023.029064

Abstract

Background: Oral squamous cell carcinoma (OSCC) is a common malignant tumor. Recently, Laminin Gamma 2 (LAMC2) has been shown to be abnormally expressed in OSCC; however, how LAMC2 signaling contributes to the occurrence and development of OSCC and the role of autophagy in OSCC has not been fully explored. This study aimed to analyze the role and mechanism of LAMC2 signaling in OSCC and the involvement of autophagy in OSCC. Methods: To explore the mechanism by which LAMC2 is highly expressed in OSCC, we used small interfering RNA (siRNA) to knock down LAMC2 to further observe the changes in the signaling pathway. Furthermore, we used cell proliferation assays, Transwell invasion assays, and wound-healing assays to observe the changes in OSCC proliferation, invasion, and metastasis. RFP-LC3 was used to detect the level of autophagy intensity. A cell line-derived xenograft (CDX) model was used to detect the effect of LAMC2 on tumor growth in vivo. Results: This study found that the level of autophagy was correlated with the biological behavior of OSCC. The downregulation of LAMC2 activated autophagy and inhibited OSCC proliferation, invasion, and metastasis via inhibiting the PI3K/AKT/mTOR pathway. Moreover, autophagy has a dual effect on OSCC, and the synergistic downregulation of LAMC2 and autophagy can inhibit OSCC metastasis, invasion, and proliferation via the PI3K/AKT/mTOR pathway. Conclusions: LAMC2 interacts with autophagy to regulate OSCC metastasis, invasion, and proliferation via the PI3K/AKT/mTOR pathway. LAMC2 down-regulation can synergistically modulate autophagy to inhibit OSCC migration, invasion, and proliferation.

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APA Style
SHAN, F., LIANG, L., FENG, C., XU, H., WANG, Z. et al. (2023). LAMC2 regulates proliferation, migration, and invasion mediated by the pl3k/akt/mtor pathway in oral squamous carcinoma. Oncology Research, 31(4), 481-493. https://doi.org/10.32604/or.2023.029064
Vancouver Style
SHAN F, LIANG L, FENG C, XU H, WANG Z, LIU W, et al. LAMC2 regulates proliferation, migration, and invasion mediated by the pl3k/akt/mtor pathway in oral squamous carcinoma. Oncol Res. 2023;31(4):481-493 https://doi.org/10.32604/or.2023.029064
IEEE Style
F. SHAN et al., "LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral squamous carcinoma," Oncol. Res., vol. 31, no. 4, pp. 481-493. 2023. https://doi.org/10.32604/or.2023.029064



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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