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YWHAH activates the HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect the proliferation of gastric cancer cells

JUNYU HE1,2,3, FENG ZENG1,2,3, XI JIN1,2,3, LIN LIANG1,2,3, MENGXIANG GAO1,2,3, WENTAO LI1,2,3, GUIYUAN LI1,2,3, YANHONG ZHOU1,2,3,*

1 NHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, China
2 Cancer Research Institute, Basic School of Medicine, Central South University, Changsha, 410078, China
3 Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, China

* Corresponding Author: YANHONG ZHOU. Email: email

Oncology Research 2023, 31(4), 615-630. https://doi.org/10.32604/or.2023.029698

Abstract

Fos-related antigen 1 (Fra-1) is a nuclear transcription factor that regulates cell growth, differentiation, and apoptosis. It is involved in the proliferation, invasion, apoptosis and epithelial mesenchymal transformation of malignant tumor cells. Fra-1 is highly expressed in gastric cancer (GC), affects the cycle distribution and apoptosis of GC cells, and participates in GC occurrence and development. However, the detailed mechanism of Fra-1 in GC is unclear, such as the identification of Fra-1-interacting proteins and their role in GC pathogenesis. In this study, we identified tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta (YWHAH) as a Fra-1-interacting protein in GC cells using co-immunoprecipitation combined with liquid chromatography-tandem mass spectrometry. Experiments showed that YWHAH positively regulated Fra-1 mRNA and protein expression, and affected GC cell proliferation. Whole proteome analysis showed that Fra-1 affected the activity of the high mobility group AT-hook 1 (HMGA1)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway in GC cells. Western blotting and flow cytometry confirmed that YWHAH activated HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect GC cell proliferation. These results will help to discover new molecular targets for the early diagnosis, treatment, and prognosis prediction of GC.

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APA Style
HE, J., ZENG, F., JIN, X., LIANG, L., GAO, M. et al. (2023). YWHAH activates the hmga1/pi3k/akt/mtor signaling pathway by positively regulating fra-1 to affect the proliferation of gastric cancer cells. Oncology Research, 31(4), 615-630. https://doi.org/10.32604/or.2023.029698
Vancouver Style
HE J, ZENG F, JIN X, LIANG L, GAO M, LI W, et al. YWHAH activates the hmga1/pi3k/akt/mtor signaling pathway by positively regulating fra-1 to affect the proliferation of gastric cancer cells. Oncol Res. 2023;31(4):615-630 https://doi.org/10.32604/or.2023.029698
IEEE Style
J. HE et al., "YWHAH activates the HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect the proliferation of gastric cancer cells," Oncol. Res., vol. 31, no. 4, pp. 615-630. 2023. https://doi.org/10.32604/or.2023.029698



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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