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GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting miRNA-566/F-box protein 47

HUALIANG LV1,#,*, CHANGCHUN LAI2,#, WENQU ZHAO3, YIBO SONG1

1 Department of Pulmonary and Critical Care Medicine, Maoming People’s Hospital, Maoming, China
2 Department of Clinical Laboratory, Maoming People’s Hospital, Maoming, China
3 Department of Pulmonary and Critical Care Medicine, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou, China

* Corresponding Author: HUALIANG LV. Email: email
# Equal contributor

Oncology Research 2021, 29(6), 401-409. https://doi.org/10.32604/or.2022.025262

Abstract

It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers. However, its expression pattern and functions in non-small cell lung cancer (NSCLC) are still largely unknown. This study aims to assess GABPB1-AS1 expression and biological roles in NSCLC. The expression of GABPB1-AS1 was detected in NSCLC specimens and adjacent normal specimens. CCK8 and Transwell assays were performed to evaluate the effects of GABPB1-AS1 on NSCLC cell proliferation, migration and invasion. Bioinformatics tools and luciferase reporter assays were applied to predict and verify GABPB1-AS1’s direct targets. The results revealed that GABPB1-AS1 is sharply reduced in NSCLC specimens and cell lines. CCK8 assays indicated that overexpression of GABPB1-AS1 dramatically reduced NSCLC cell growth, and Transwell assays proved that NSCLC cell migration and invasion were distinctly inhibited by GABPB1-AS1. Exploration of the mechanism uncovered that miRNA-566 (miR-566)/F-box protein 47 (FBXO47) is directly targeted by GABPB1-AS1 in NSCLC. The study demonstrated that GABPB1-AS1 inhibited NSCLC cell proliferation, migration and invasion by targeting miR-566/FBXO47.

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APA Style
LV, H., LAI, C., ZHAO, W., SONG, Y. (2021). GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting mirna-566/f-box protein 47. Oncology Research, 29(6), 401-409. https://doi.org/10.32604/or.2022.025262
Vancouver Style
LV H, LAI C, ZHAO W, SONG Y. GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting mirna-566/f-box protein 47. Oncol Res. 2021;29(6):401-409 https://doi.org/10.32604/or.2022.025262
IEEE Style
H. LV, C. LAI, W. ZHAO, and Y. SONG "GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting miRNA-566/F-box protein 47," Oncol. Res., vol. 29, no. 6, pp. 401-409. 2021. https://doi.org/10.32604/or.2022.025262



cc Copyright © 2021 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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