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LncRNA PRRT3-AS1 exerts oncogenic effects on nonsmall cell lung cancer by targeting microRNA-507/homeobox B5 axis

RUI ZHOU#, JIANYANG XU#, LINGWEI WANG*, JIANXIN LI*

Department of Thoracic Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116011, China

* Corresponding Authors: LINGWEI WANG. Email: email; JIANXIN LI. Email: email

Oncology Research 2021, 29(6), 411-423. https://doi.org/10.32604/or.2022.026236

Abstract

Long noncoding RNAs (lncRNAs) act as key regulators controlling complex cellular behaviors in nonsmall cell lung cancer (NSCLC). We investigated the expression of lncRNA PRRT3 antisense RNA 1 (PRRT3-AS1) in paired samples of NSCLC and adjacent normal tissues from a patient cohort in our hospital using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and found that it was significantly higher in NSCLC tissue than in normal tissue, consistent with The Cancer Genome Atlas database. Furthermore, functional investigation revealed that lncRNA PRRT3-AS1 depletion inhibited NSCLC-cell proliferation, colony formation, invasion, and migration, whereas its overexpression exerted the opposite effects. Moreover, PRRT3-AS1 knockdown suppressed in vivo NSCLC growth. Investigation of downstream mechanisms using RNA immunoprecipitation and luciferase reporter assay revealed that lncRNA PRRT3-AS1 acted as a competing endogenous RNA by adsorbing microRNA-507 (miR-507) and enhanced the expression of its target gene, homeobox B5 (HOXB5), in NSCLC. Furthermore, miR-507 downregulation or HOXB5 upregulation eliminated the cancer-inhibiting effects of lncRNA PRRT3-AS1 depletion in NSCLC cells. To conclude, the lncRNA PRRT3-AS1/miR-507/HOXB5 pathway acts as a promoter of malignant characteristics in NSCLC, and this newly identified competing endogenous RNA pathway may be an effective diagnostic, prognostic, and therapeutic target in NSCLC.

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APA Style
ZHOU, R., XU, J., WANG, L., LI, J. (2021). Lncrna PRRT3-AS1 exerts oncogenic effects on nonsmall cell lung cancer by targeting microrna-507/homeobox B5 axis. Oncology Research, 29(6), 411-423. https://doi.org/10.32604/or.2022.026236
Vancouver Style
ZHOU R, XU J, WANG L, LI J. Lncrna PRRT3-AS1 exerts oncogenic effects on nonsmall cell lung cancer by targeting microrna-507/homeobox B5 axis. Oncol Res. 2021;29(6):411-423 https://doi.org/10.32604/or.2022.026236
IEEE Style
R. ZHOU, J. XU, L. WANG, and J. LI "LncRNA PRRT3-AS1 exerts oncogenic effects on nonsmall cell lung cancer by targeting microRNA-507/homeobox B5 axis," Oncol. Res., vol. 29, no. 6, pp. 411-423. 2021. https://doi.org/10.32604/or.2022.026236



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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