Open Access
ARTICLE
Identification of lncRNAs associated with the progression of acute lymphoblastic leukemia using a competing endogenous RNAs network
SHAHRAM NEKOEIAN1, TAHEREH ROSTAMI2, AMIR NOROUZY3, SAFIN HUSSEIN1,4, GHOLAMREZA TAVOOSIDANA1, BAHRAM CHAHARDOULI2, SHAHRBANO ROSTAMI2,*, YAZDAN ASGARI5, ZAHRA AZIZI1,*
1 Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2 Research Institute for Oncology, Hematology and Cell Therapy, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Energy & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
4 Department of Medical Laboratory Science, University of Raparin, Rania, Kurdistan Region, Iraq
5 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
* Corresponding Authors: Zahra Azizi, ; Shahrbano Rostami,
Oncology Research 2022, 30(6), 259-268. https://doi.org/10.32604/or.2022.027904
Received 20 November 2022; Accepted 01 February 2023; Issue published 09 February 2023
Abstract
Acute lymphoblastic leukemia (ALL) is a malignancy of bone marrow lymphoid precursors. Despite effective
treatments, the causes of its progression or recurrence are still unknown. Finding prognostic biomarkers is needed for
early diagnosis and more effective treatment. This study was performed to identify long non-coding RNAs (lncRNAs)
involved in ALL progression by constructing a competitive endogenous RNA (ceRNA) network. These lncRNAs may
serve as potential new biomarkers in the development of ALL. The GSE67684 dataset identified changes in lncRNAs
and mRNAs involved in ALL progression. Data from this study were re-analyzed, and probes related to lncRNAs
were retrieved. Targetscan, miRTarBase, and miRcode databases were used to identify microRNAs (miRNAs) related
to the discovered genes and lncRNAs. The ceRNA network was constructed, and the candidate lncRNAs were
selected. Finally, the results were validated with reverse transcription quantitative real-time PCR (RT-qPCR). The
ceRNA network outcomes demonstrated that the top lncRNAs associated with altered mRNAs in ALL are IRF1-AS1,
MCM3AP-AS1, TRAF3IP2-AS1, HOTAIRM1, CRNDE, and TUG1. Investigations of the subnets linked to
MCM3AP-AS1, TRAF3IP2-AS1, and IRF1-AS1 indicated that these lncRNAs were considerably related to pathways
associated with inflammation, metastasis, and proliferation. Higher expression levels of IRF1-AS1, MCM3AP-AS1,
TRAF3IP2-AS1, CRNDE, and TUG1 were found in ALL samples compared to controls. The expression of MCM3APAS1, TRAF3IP2-AS1, and IRF1-AS1 is significantly elevated during the progression of ALL, playing an oncogenic
role. Due to their role in the main cancer pathways, lncRNAs could be suitable therapeutic and diagnostic targets in ALL.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
NEKOEIAN, S., ROSTAMI, T., NOROUZY, A., HUSSEIN, S., TAVOOSIDANA, G. et al. (2022). Identification of lncRNAs associated with the progression of acute lymphoblastic leukemia using a competing endogenous RNAs network.
Oncology Research, 30(6), 259–268. https://doi.org/10.32604/or.2022.027904