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ARTICLE
Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola (Brassica napus L.)
Reza Shokri-Gharelo1, Ali Bandehagh1,*, Mohammad Anwar Hossain2,*
1
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, 51368, Iran
2
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
* Corresponding Authors: Ali Bandehagh. Email: ; Mohammad Anwar Hossain. Email:
Phyton-International Journal of Experimental Botany 2022, 91(7), 1403-1417. https://doi.org/10.32604/phyton.2022.020431
Received 23 November 2021; Accepted 17 January 2022; Issue published 14 March 2022
Abstract
Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought
conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants
experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola
(
Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to
drought stress. The experiment used a completely randomized design, implemented in a hydroponic system under
greenhouse conditions. To impose drought stress, plants were exposed to Hoagland’s solution supplemented with
polyethylene glycol (PEG) 6000 for 7 days. The drought stress resulted in 161reproducible protein spots in twodimensional electrophoresis of canola leaves. The t-student test showed 21 differentially abundant proteins
(DAP), of which 2 and 19 were up and down accumulated, respectively. Two spots identified as 1-aminocyclopropane-1-carboxylate oxidase and D-2-hydroxyglutarate dehydrogenase showed an increased abundance of 2.11 and
1.77, respectively. The extended protein-protein interaction of differentially abundant proteins and KEGG analysis
showed 47 pathways directly and indirectly associated with canola response to drought stress. DAPs with increased
abundance were associated with amino acid and signaling processes, whereas DAPs with decreased abundance were
mostly connected with pathways responsible for energy production. The results of the study will help to elucidate
further the molecular events associated with the susceptibility to drought stress in canola.
Keywords
Cite This Article
Shokri-Gharelo, R., Bandehagh, A., Hossain, M. A. (2022). Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola (
Brassica napus L.).
Phyton-International Journal of Experimental Botany, 91(7), 1403–1417.