Open Access
ARTICLE
Identification and Characterization of a Novel Yellow Leaf Mutant yl1 in Rice
Xiaofang Zeng1,#, Guangzheng Li1,#, Nu’an Liu2, Yan Li1, Jianrong Li1, Xiaozhen Huang1, Degang Zhao1,2,,*
1
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education,
Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, 550025, China
2
Guizhou Plant Conservation Technology Center, Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of
Agricultural Sciences, Guiyang, 550006, China
* Corresponding Author: Degang Zhao. Email:
# These authors contributed equally to this study
(This article belongs to the Special Issue: High-Yield Rice Physiology & Genetics)
Phyton-International Journal of Experimental Botany 2022, 91(11), 2419-2437. https://doi.org/10.32604/phyton.2022.021199
Received 02 January 2022; Accepted 03 March 2022; Issue published 12 July 2022
Abstract
Leaf-color mutants play an important role in the study of chlorophyll metabolism, chloroplast development, and
photosynthesis system. In this study, the
yellow leaf 1 (
yl1) rice mutant was identified from the ethyl methane
sulfonate-treated mutant progeny of Lailong, a glutinous
japonica rice landrace cultivated in Guizhou Province,
China. Results showed that
yl1 exhibited yellow leaves with decreased chlorophyll content throughout the growth
period. Chloroplast development in the
yl1 mutant was disrupted, and the grana lamellae was loosely packed and
disordered. RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) analysis revealed
that the chlorophyll synthesis-related genes
OsCHLH,
OsCHLM,
OsCHLG,
PORB, and
YGL8, as well as the chloroplast development-related genes
FtsZ,
OsRpoTp, and
RbcL, were down-regulated in the
yl1 mutant. Genetic analysis revealed that the yellow leaf phenotype of
yl1 was controlled by recessive nuclear gene. By employing the
MutMap method, the mutation responsible for the phenotype was mapped to a 6.17 Mb region between
17.34 and 23.51 Mb on chromosome 3. Two non-synonymous single-nucleotide polymorphisms (SNPs) located
in the gene locus
LOC_Os03g31210 and
LOC_Os03g36760 were detected in this region. The two SNPs were
further confirmed by PCR and Sanger sequencing. The expression patterns of the two candidate genes indicated
that
LOC_Os03g36760 showed greater potential for functional verification. Subcellular protein localization
revealed that the encoded product of
LOC_Os03g36760 was localized in the nucleus, cytoplasm, and plasma membrane. These results will be useful for further characterization and cloning of the
yl1 gene, and for research on the
molecular mechanisms controlling biogenesis and chloroplast biochemical processes.
Keywords
Cite This Article
Zeng, X., Li, G., Liu, N., Li, Y., Li, J. et al. (2022). Identification and Characterization of a Novel Yellow Leaf Mutant
yl1 in Rice.
Phyton-International Journal of Experimental Botany, 91(11), 2419–2437. https://doi.org/10.32604/phyton.2022.021199