Open Access
ARTICLE
Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth
Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*
1
College of Agronomy, Jilin Agricultural University, Changchun, 130118, China
2
College of Life Science, Jilin Agricultural University, Changchun, 130118, China
3
Department of Biology, College of Natural and Computational Science, Wachemo University, Hossana, 667, Ethiopia
* Corresponding Authors: Abraham Lamboro. Email: ; Jun Zhang. Email:
Phyton-International Journal of Experimental Botany 2023, 92(9), 2489-2501. https://doi.org/10.32604/phyton.2023.029492
Received 22 February 2023; Accepted 26 May 2023; Issue published 28 July 2023
Abstract
The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was
tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main
growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied
in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in
the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at higher
concentrations where shooting rates were not affected by the treatments. In cefotaxime, timentin, and carbenicillin-containing media, levels of antibiotics greater than 400, 300, and 200 mg/L, respectively resulted in the brown
coloration of plantlets. Moreover, the mean shoot number and shoot weight significantly decreased as their dosage
increased. The results indicate that maximum levels of antibiotics have an adverse effect on the growth and development of peanuts. Also, cefotaxime (100–300 mg/L) and timentin (100–300 mg/L) will be sufficient in controlling
Agrobacterium growth in the culture media with the least phytotoxic effect on the peanut plants.
Keywords
Cite This Article
APA Style
Lamboro, A., Yang, S., Li, X., Yao, D., Zhang, J. (2023). Evaluation of beta-lactam antibiotics on the regeneration of peanut plants and their inhibitory effect on <i>agrobacterium</i> growth. Phyton-International Journal of Experimental Botany, 92(9), 2489-2501. https://doi.org/10.32604/phyton.2023.029492
Vancouver Style
Lamboro A, Yang S, Li X, Yao D, Zhang J. Evaluation of beta-lactam antibiotics on the regeneration of peanut plants and their inhibitory effect on <i>agrobacterium</i> growth. Phyton-Int J Exp Bot. 2023;92(9):2489-2501 https://doi.org/10.32604/phyton.2023.029492
IEEE Style
A. Lamboro, S. Yang, X. Li, D. Yao, and J. Zhang "Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on <i>Agrobacterium</i> Growth," Phyton-Int. J. Exp. Bot., vol. 92, no. 9, pp. 2489-2501. 2023. https://doi.org/10.32604/phyton.2023.029492