Table of Content

Open Access

ARTICLE

Apparent cross-talk of two signaling pathways that regulate Zea mays coleoptile growth

Buentello Volante1 B, F Díaz de León-Sánchez1, F Rivera-Cabrera1, R Aguilar Caballero2, M Ponce-Valadez1, E Sánchez de Jiménez2, LJ Pérez-F lores1
Departamento de Ciencias de la Salud Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186 Col. Vicentina Iztapalapa 09270 México, D.F.
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México. Circuito Institutos, Ciudad Universitaria 04510 México, D.F.
Address Correspondence to: Dra. Laura Josefina Pérez-F lores, email: ljpf@xanum.uam.mx; fax 052-55-58044727, phone 052-55-58046481 and E. Sánchez de Jiménez, email: estelas@servidor.unam.mx; Fax 052-55-56225329, phone 052-55-56225278.

Phyton-International Journal of Experimental Botany 2010, 79(all), 101-108. https://doi.org/10.32604/phyton.2010.79.101

Abstract

Auxin and insulin promote Zea mays embryo growth, induce S6 ribosomal protein (S6rp) phosphorylation, and promote specific protein synthesis. The objective of this research was to test a possible cross-talk between insulin and auxin transduction pathways in Z. mays coleoptiles, typical auxin target tissues. Auxin and insulin produced differential quantitative and qualitative stimulation of cytoplasmic and ribosomal protein phosphorylation, and specific patterns of de novo synthesized cytoplasmic proteins. In addition, insulin induced S6rp phosphorylation was strongly inhibited by rapamycin, indicating target of rapamycin (TOR) kinase participation; auxin-induced S6rp phosphorylation was insensitive to this inhibitor. Phosphatidic acid (PA), a second messenger of TOR in metazoan, was also tested. It produced similar results to insulin and rapamycin sensitiveness, supporting the existence of TOR pathway in plants and the participation of PA as an intermediate of insulin action. These results seem to imply that auxin and insulin induce Z. mays coleoptile growth through two independent signal transduction pathways.

Keywords

Auxins, Insulin, Phosphatidic acid, ZmS6K, TOR, Plant growth.

Cite This Article

Volante, B., Rivera-Cabrera, F., Caballero, R. A., Ponce-Valadez, M., Sánchez, E. et al. (2010). Apparent cross-talk of two signaling pathways that regulate Zea mays coleoptile growth. Phyton-International Journal of Experimental Botany, 79(all), 101–108.

Citations




This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 870

    View

  • 575

    Download

  • 1

    Like

Share Link

WeChat scan