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Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley

Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue*, Xiaoqin Zhang*
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China
* Corresponding Authors: Dawei Xue. Email: ; Xiaoqin Zhang. Email:

Phyton-International Journal of Experimental Botany 2020, 89(2), 229-251. https://doi.org/10.32604/phyton.2020.10022

Received 05 February 2020; Accepted 29 February 2020; Issue published 22 April 2020

Abstract

The F-box protein-encoding gene family plays an essential role in plant stress resistance. In present study, 126 non-redundant F-box genes were identified in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165– 887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana (At) was used to classify barley F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs. In total, 124 F-box genes were unevenly distributed on 7 chromosomes; another 2 genes have not been anchored yet. The gene structure analysis revealed high variability in the number of exons and introns in F-box genes. Comprehensive analysis of expression profiles and phylogenetic tree analysis, a total of 12 F-box genes that may be related to stress tolerance in barley were screened. Of the 12 detected F-box genes, 8 and 10 were upregulated after drought and salt stress treatments, respectively, using quantitative real-time polymerase chain reaction (qRT-PCR). This study is the first systematic analysis conducted on the F-box gene family in barley, which is of great importance for clarifying this family’s bioinformatic characteristics and elucidating its function in barley stress resistance. These results will serve as a theoretical reference for subsequent research on molecular regulation mechanisms, genetic breeding, and improvement.

Keywords

Drought and salt stress; barley; expression analysis; F-box gene family; phylogenetic analysis

Cite This Article

Zhang, L., Wang, S., Chen, Y., Dong, M., Fang, Y. et al. (2020). Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley. Phyton-International Journal of Experimental Botany, 89(2), 229–251.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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