Vol.90, No.2, 2021, pp.475-487, doi:10.32604/phyton.2021.013557
Doubled Haploid Production Using an Improved Anther Culture Protocol for Sorghum [Sorghum bicolor (L.) Moench]
  • Paul Chege1,2, Erzsébet Kiss2, Csaba Lantos1, Andrea Palágyi1, János Pauk1,2,*
1 Cereal Research Non-profit Ltd., Alsó kikötő sor 9, Szeged, 6726, Hungary
2 Doctoral School of Plant Science, Szent István University, Gödöllő H, 2103, Hungary
* Corresponding Author: János Pauk. Email:
Received 11 August 2020; Accepted 19 October 2020; Issue published 07 February 2021
Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F1 registered- and experimental hybrids involved, this study successfully produced haploids from five genotypes and subsequently, four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl−1 L-proline, 1.0 gl−1 L-asparagine and 1.0 gl−1 KH2PO4. Medium 190-2Cu was used for regeneration and rooting, which occurred successfully, if the calli were transferred on to it less than 7 days after induction, and temperature was maintained at 25˚C under light condition. Genotype dependency was not wholly overcome; however, sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets. Spontaneous diploids producing seeds at rates of upto 80.5% were obtained, therefore eliminating the need for colchicine duplication.
In vitro androgenesis; doubled-haploid; sorghum; anther-culture; breeding
Cite This Article
Chege, P., Kiss, E., Lantos, C., Palágyi, A., Pauk, J. (2021). Doubled Haploid Production Using an Improved Anther Culture Protocol for Sorghum [Sorghum bicolor (L.) Moench]. Phyton-International Journal of Experimental Botany, 90(2), 475–487.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.