Open Access
ARTICLE
In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria
Roberto Arredondo-Valdés1, Francisco D. Hernández-Castillo2, Mario Rocandio-Rodríguez1, Julia C. Anguiano-Cabello3, Madai Rosas-Mejía1, Venancio Vanoye-Eligio1, Salvador Ordaz-Silva4, Imelda V. López-Sánchez4, Laura D. Carrazco-Peña4, Julio C. Chacón-Hernández1,*
1 Institute of Applied Ecology, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, 87019, México
2 Parasitology Department, Universidad Autónoma Agraria Antonio Narro, Saltillo, 25315, México
3 Health Area, Universidad La Salle Saltillo, Saltillo, 25298, México
4 Faculty of Engineering and Business San Quintin, Universidad Autónoma de Baja California, San Quintín, 22930, México
* Corresponding Author: Julio C. Chacón-Hernández. Email:
(This article belongs to this Special Issue: Plant Secondary Metabolites: Recent Advances and Opportunities)
Phyton-International Journal of Experimental Botany 2021, 90(3), 895-906. https://doi.org/10.32604/phyton.2021.014301
Received 16 September 2020; Accepted 04 January 2020; Issue published 30 March 2021
Abstract
The tomato (
Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like
Agrobacterium tumefeciens (
At),
Clavibacter
michiganensis subsp.
michiganensis (
Cmm),
Pseudomonas syringae pv. tomato (
Pst),
Ralstonia solanacearum (
Rs),
and
Xanthomonas axonopodis (
Xa). Synthetic chemical products are used mostly on disease plant control, but
overuse generates resistance to bacterial control. This study aimed to evaluate the
in vitro antibacterial activity
of the ethanolic extract of
Moringa oleifera Lam. leaves against
At,
Cmm,
Pst,
Rs, and
Xa, as well as information
about this plant species’ chemical composition. Antibacterial activity against pathogens observed by microplate
technique, phytochemical screening, and FTIR analysis revealed different bio-active compounds on ethanolic
extracts with antibacterial activity. The growth inhibition rate ranged between 0.08% and 99.94%. The inhibitory
concentration, IC50, required to inhibit 50% of
At,
Cmm,
Pst,
Rs, and
Xa bacterial growth, was 276.67, 350.48,
277.85, 351.49, and 283.22 mg/L, respectively. Inhibition of phytopathogen bacteria’s growth increased as the concentrations of the extract also increased.
Moringa oleifera extract can be recommended as a potent bio-bactericide.
Keywords
Cite This Article
Arredondo-Valdés, R., Hernández-Castillo, F. D., Rocandio-Rodríguez, M., Anguiano-Cabello, J. C., Rosas-Mejía, M. et al. (2021).
In vitro Antibacterial Activity of
Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria.
Phyton-International Journal of Experimental Botany, 90(3), 895–906. https://doi.org/10.32604/phyton.2021.014301
Citations