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ARTICLE
Differential Responses of NHX1 and SOS1 Gene Expressions to Salinity in two Miscanthus sinensis Anderss. Accessions with Different Salt Tolerance
Qian Sun1,2, Toshihiko Yamada3, Yulai Han2,*, Tetsuo Takano1,*
1 Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo, 188-0002, Japan
2 College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, 518118, China
3 Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, 060-0811, Japan
* Corresponding Authors: Yulai Han. Email: ; Tetsuo Takano. Email: -tokyo.ac.jp
Phyton-International Journal of Experimental Botany 2021, 90(3), 827-836. https://doi.org/10.32604/phyton.2021.013805
Received 20 August 2020; Accepted 01 December 2020; Issue published 30 March 2021
Abstract
The lignocellulosic crop
Miscanthus spp. has been identified as a good candidate for biomass production. The
responses of
Miscanthus sinensis Anderss. to salinity were studied to satisfy the needs for high yields in marginal
areas and to avoid competition with food production. The results indicated that the relative advantages of the
tolerant accession over the sensitive one under saline conditions were associated with restricted Na
+ accumulation
in shoots. Seedlings of two accessions (salt-tolerant ‘JM0119’ and salt-sensitive ‘JM0099’) were subjected to 0
(control), 100, 200, and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes
involved in Na
+ accumulation in
M. sinensis. The adaptation responses of genes encoding for Na
+
/H
+ antiporters,
NHX1 and
SOS1 to NaCl stress were examined in JM0119 and JM0099.The cDNA sequences of genes examined
were highly conserved among the relatives of
M. sinensis based on the sequencing on approximate 600 bp-long
cDNA fragments obtained from degenerate PCR. These salt-induced variations of gene expression investigated by
quantitative real-time PCR provided evidences for insights of the molecular mechanisms of salt tolerance in
M. sinensis. The expression of
NHX1 was up-regulated by salt stress in JM0119 shoot and root tissues. However,
it was hardly affected in JM0099 shoot tissue except for a significant increase at the 100 mM salt treatment, and it
was salt-suppressed in the JM0099 root tissue. In the root tissue, the expression of
SOS1 was induced by the high
salt treatment in JM0119 but repressed by all salt treatments in JM0099. Thus, the remarkably higher expression
of
NHX1 and
SOS1 were associated with the resistance to Na
+ toxicity by regulation of the Na
+ influx, efflux, and
sequestration under different salt conditions.
Keywords
Cite This Article
APA Style
Sun, Q., Yamada, T., Han, Y., Takano, T. (2021). Differential responses of <i>nhx1</i> and <i>sos1</i> gene expressions to salinity in two <i>miscanthus sinensis</i> anderss. accessions with different salt tolerance. Phyton-International Journal of Experimental Botany, 90(3), 827-836. https://doi.org/10.32604/phyton.2021.013805
Vancouver Style
Sun Q, Yamada T, Han Y, Takano T. Differential responses of <i>nhx1</i> and <i>sos1</i> gene expressions to salinity in two <i>miscanthus sinensis</i> anderss. accessions with different salt tolerance. Phyton-Int J Exp Bot. 2021;90(3):827-836 https://doi.org/10.32604/phyton.2021.013805
IEEE Style
Q. Sun, T. Yamada, Y. Han, and T. Takano "Differential Responses of <i>NHX1</i> and <i>SOS1</i> Gene Expressions to Salinity in two <i>Miscanthus sinensis</i> Anderss. Accessions with Different Salt Tolerance," Phyton-Int. J. Exp. Bot., vol. 90, no. 3, pp. 827-836. 2021. https://doi.org/10.32604/phyton.2021.013805
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