Open Access

ARTICLE

Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

Longhua Zhou^1,2,#, Ting He^1,2,#, Jing Li^1,2,3, Guimei Guo^1,2, Yingbo Li^1,2, Hongwei Xu^1,2, Runhong Gao^1,2, Linli Huang^1,2, Yifei Wang^1,2, Ruiju Lu^1,2, Zhiwei Chen^1,2,*, Chenghong Liu^1,2,*

1 Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 200000, China
2 Biotechnology Research Institute, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, 200000, China
3 Shanghai Baixin Biotechnology Company Limited, Shanghai, 200000, China

* Corresponding Authors: Zhiwei Chen. Email: ; Chenghong Liu. Email:

Phyton-International Journal of Experimental Botany 2022, 91(7), 1419-1428. https://doi.org/10.32604/phyton.2022.019912

Received 23 October 2021; Accepted 22 December 2021; Issue published 14 March 2022

Abstract

Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and roots using LC-MS (liquid chromatography mass spectrometry). By comparing the shoots of the haploid and diploid plants, it was found that the distribution trend of the six phytohormones was consistent, and another consistent trend was found in the roots of the diploid and tetraploid plants. In addition, we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid, diploid and tetraploid. Here, the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.

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Keywords

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