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ARTICLE
The Cotton GhWRKY91 Gene Negatively Regulates Root Elongation in Overexpressed Transgenic Arabidopsis thaliana
Yueying Liu#, Yuqing Wang#, Lijiao Gu*
College of Forestry, Hebei Agricultural University, Baoding, 071000, China
* Corresponding Author: Lijiao Gu. Email:
# These authors contributed equally to this work
(This article belongs to the Special Issue: Abiotic and Biotic Stress Tolerance in Crop)
Phyton-International Journal of Experimental Botany 2023, 92(11), 2937-2946. https://doi.org/10.32604/phyton.2023.043306
Received 28 June 2023; Accepted 06 August 2023; Issue published 24 October 2023
Abstract
WRKY transcription factors play important roles in plant growth, development, and stress responses. Our previous research has shown that the
GhWRKY91 gene can delay age-, abscisic acid (ABA)-, and drought-induced
leaf senescence when overexpressed in transgenic
Arabidopsis plants. To explore in more depth the biological
functions of the
GhWRKY91 gene, we further observed the root growth of overexpressing transgenic
Arabidopsis thaliana under ABA and drought treatment. In this study, we transplanted the germinated seeds of wild-type
(WT) and three transgenic lines (OE-12, OE-13 and OE-20) to 1/2 MS solid medium containing ABA and different concentrations of mannitol (simulated drought treatment) for culturing. The results showed that the transgenic plants had dark green leaves and short root lengths when no stress treatment was added. After ABA and
mannitol treatment, the root growth of the WT and transgenic
Arabidopsis was inhibited to varying degrees, and
the root length downregulation of the transgenic plants was higher than that of the WT, indicating that they were
more sensitive to ABA and drought. A bimolecular fluorescence complementation (BiFC) assay showed that the
GhWRKY91 and
GhWRKY3 proteins interact and emit yellow fluorescence in tobacco leaf cells. These results
indicate that the
GhWRKY91 gene negatively regulates root elongation in transgenic
Arabidopsis and provide a
basis for further research on the molecular mechanism of its involvement in regulating cotton root development.
Keywords
Cite This Article
APA Style
Liu, Y., Wang, Y., Gu, L. (2023). The cotton <i>ghwrky91</i> gene negatively regulates root elongation in overexpressed transgenic <i>arabidopsis thaliana</i>. Phyton-International Journal of Experimental Botany, 92(11), 2937-2946. https://doi.org/10.32604/phyton.2023.043306
Vancouver Style
Liu Y, Wang Y, Gu L. The cotton <i>ghwrky91</i> gene negatively regulates root elongation in overexpressed transgenic <i>arabidopsis thaliana</i>. Phyton-Int J Exp Bot. 2023;92(11):2937-2946 https://doi.org/10.32604/phyton.2023.043306
IEEE Style
Y. Liu, Y. Wang, and L. Gu "The Cotton <i>GhWRKY91</i> Gene Negatively Regulates Root Elongation in Overexpressed Transgenic <i>Arabidopsis thaliana</i>," Phyton-Int. J. Exp. Bot., vol. 92, no. 11, pp. 2937-2946. 2023. https://doi.org/10.32604/phyton.2023.043306