Open Access
ARTICLE
Overexpression of the LcPIN2 and LtPIN2 Gene in Arabidopsis thaliana Promotes Root Elongation
Zijian Cao#, Guoxia Xue#, Lingfeng Hu, Haoxian Qu, Shuang Liang, Jisen Shi, Jinhui Chen*, Zhaodong Hao*
Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China
* Corresponding Authors: Jinhui Chen. Email: ; Zhaodong Hao. Email:
Phyton-International Journal of Experimental Botany 2023, 92(8), 2383-2397. https://doi.org/10.32604/phyton.2023.029845
Received 10 March 2023; Accepted 26 April 2023; Issue published 25 June 2023
Abstract
The auxin polar transporter, PIN-FORMED 2 (PIN2) plays an important role in root development. However, it remains unclear whether
PIN2 genes form two
Liriodendron species,
L. chinense (LcPIN2) and
L. tulipifera (LtPIN2), are both involved in root development and whether and to what extent these two genes diverge in function. Here, we cloned and overexpressed
LcPIN2 and
LtPIN2 in A
rabidopsis thaliana wild-type (WT) and
Atpin2 mutant. Phylogenetic and sequence analysis showed a small degree of differentiation between these two
Liriodendron PIN2 genes. Tissue-specific gene expression analysis indicated that both
Liriodendron PIN2 genes were highly expressed in roots, implying a potential role in root development. Finally, heterologous overexpression of
LcPIN2 and
LtPIN2 in
Arabidopsis both significantly increased the root length compared to wild-type and empty vector. Furthermore, the root length defect in
Atpin2 was complemented both by
LcPIN2 and
LtPIN2. However, heterologous overexpression of
LcPIN2 and
LtPIN2 cannot rescue the defect in root gravitropism of
Atpin2 mutants. Taken together, our findings unravel
PIN2 genes from the magnoliids plant
Liriodendron were functionally conserved with AtPIN2 in the dicotyledonous plant
Arabidopsis in regard to the regulation of root length, but not root gravitropism. This study also provides a potential target for genetic improvement of the root system in these valuable forest trees
Liriodendron.
Keywords
Cite This Article
APA Style
Cao, Z., Xue, G., Hu, L., Qu, H., Liang, S. et al. (2023). Overexpression of the <i>lcpin2</i> and <i>ltpin2</i> gene in <i>arabidopsis thaliana</i> promotes root elongation. Phyton-International Journal of Experimental Botany, 92(8), 2383-2397. https://doi.org/10.32604/phyton.2023.029845
Vancouver Style
Cao Z, Xue G, Hu L, Qu H, Liang S, Shi J, et al. Overexpression of the <i>lcpin2</i> and <i>ltpin2</i> gene in <i>arabidopsis thaliana</i> promotes root elongation. Phyton-Int J Exp Bot. 2023;92(8):2383-2397 https://doi.org/10.32604/phyton.2023.029845
IEEE Style
Z. Cao et al., "Overexpression of the <i>LcPIN2</i> and <i>LtPIN2</i> Gene in <i>Arabidopsis thaliana</i> Promotes Root Elongation," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2383-2397. 2023. https://doi.org/10.32604/phyton.2023.029845