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The Physiological Mechanisms Underlying N2-Fixing Common Bean (Phaseolus vulgaris L.) Tolerance to Iron Deficiency

Abdelmajid Krouma1,2,*

1 Faculty of Sciences and Techniques, University of Kairouan, Sidi Bouzid, 9100, Tunisia
2 Laboratory of Ecosystems and Biodiversity in Arid Land of Tunisia, Faculty of Sciences, University of Sfax, Sfax, Tunisia

* Corresponding Author: Abdelmajid Krouma. Email: email-kairouan.tn

(This article belongs to the Special Issue: Abiotic and Biotic Stress Tolerance in Crop)

Phyton-International Journal of Experimental Botany 2023, 92(7), 2133-2150. https://doi.org/10.32604/phyton.2023.029048

Abstract

Iron is an essential element for plants as well as all living organisms, functioning in various physiological and biochemical processes such as photosynthesis, respiration, DNA synthesis, and N2 fixation. In the soil, Fe bioavailability is extremely low, especially under aerobic conditions and at high pH ranges. In contrast, plants with nodules on their roots that fix atmospheric nitrogen need much more iron. To highlight the physiological traits underlying the tolerance of N2-fixing common bean to iron deficiency, two genotypes were hydroponically cultivated in a greenhouse: Coco nain (CN) and Coco blanc (CB). Plants were inoculated with an efficient strain of Rhizobium tropici, CIAT899, and received a nutrient solution added with 0 µM Fe (severe Fe deficiency, SFeD), 5 µM Fe (moderate Fe deficiency, MFeD) or 45 µM Fe (control, C). Several physiological parameters related to photosynthesis and symbiotic nitrogen fixation were then analyzed. Iron deficiency significantly reduced whole plant and nodule growth, chlorophyll biosynthesis, photosynthesis, leghemoglobin (LgHb), nitrogenase (N2ase) activity, nitrogen, and Fe nutrition, with some genotypic differences. As compared to CB, CN maintained better Fe allocation to shoots and nodules, allowing it to preserve the integrity of its photosynthetic and symbiotic apparatus, thus maintaining the key functional traits of the plant metabolism (chlorophyll biosynthesis and photosynthesis in shoots, leghemoglobin accumulation, and nitrogenase activity in root nodules). Plant growth depends on photosynthesis, which needs to be supplied with sufficient iron and nitrogen. Fe deficiency stress index (FeD-SI) and Fe use efficiency (FeUE) are two physiological traits of tolerance that discriminated the studied genotypes.

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APA Style
Krouma, A. (2023). The physiological mechanisms underlying n<sub>2</sub>-fixing common bean (<i>phaseolus vulgaris</i> L.) tolerance to iron deficiency. Phyton-International Journal of Experimental Botany, 92(7), 2133-2150. https://doi.org/10.32604/phyton.2023.029048
Vancouver Style
Krouma A. The physiological mechanisms underlying n<sub>2</sub>-fixing common bean (<i>phaseolus vulgaris</i> L.) tolerance to iron deficiency. Phyton-Int J Exp Bot. 2023;92(7):2133-2150 https://doi.org/10.32604/phyton.2023.029048
IEEE Style
A. Krouma, "The Physiological Mechanisms Underlying N<sub>2</sub>-Fixing Common Bean (<i>Phaseolus vulgaris</i> L.) Tolerance to Iron Deficiency," Phyton-Int. J. Exp. Bot., vol. 92, no. 7, pp. 2133-2150. 2023. https://doi.org/10.32604/phyton.2023.029048



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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