Open Access
ARTICLE
Biological Analysis and Response to Low Phosphate Stress of Phosphate Transporter Family 1 (PHT1)) Genes in Solanum tuberosum L.
Yangyang Du1, Yuanyuan Bao2, Lu Zheng1, Qinglong Tian1, Xinyong Zhang1,*
1 Key Laboratory of Crop Germplasm Innovation and Sustainable Utilization, Collage of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China
2 Laboratory of Fruit and Vegetable Preservation and Processing, College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, China
* Corresponding Author: Xinyong Zhang. Email:
Phyton-International Journal of Experimental Botany 2023, 92(8), 2325-2348. https://doi.org/10.32604/phyton.2023.027642
Received 07 November 2022; Accepted 07 April 2023; Issue published 25 June 2023
Abstract
Inorganic phosphate (Pi) is one of the main nutrients necessary for plant growth. Phosphate transporters mediate
the acquisition, transport and recycling of phosphate, which is essential for plant growth and development.
Although
PHT1 has been reported in many plants at home and abroad, it is rarely studied in potato. Therefore,
it is of great significance to study the
PHT1 family members in order to understand the molecular response
mechanism of potato in low phosphate state. In this study, a total of 6 potato
PHT1 genes were identified and
isolated. It was found that after the expression of different members of potato
PHT1 gene, there were certain
differences in amino acids and proteins, and the transmembrane domains ranged from 6 to 12. The difference
in the secondary and tertiary protein structure of potato
PHT1 also led to a difference in protein morphology.
In addition, the expression of the
PHT1 gene in potato increased obviously during 3~9 h of Pi deficiency stress.
Overall, the expression levels of different genes in roots, stems and leaves are distinctly different, but the expression levels of the
StPHT1;6 and
StPHT1;10 genes are very high in roots, stems and leaves, indicating that these two
genes may participate in the absorption of Pi in potato and play a role in Pi translocation. These two genes play a
major role in the regulation of expression under short-term Pi deficiency stress. Our results provide an important
reference for further understanding the evolution and function of potato phosphate transporters, and have important significance for improving the ability of potato to tolerate low Pi.
Keywords
Cite This Article
APA Style
Du, Y., Bao, Y., Zheng, L., Tian, Q., Zhang, X. (2023). Biological analysis and response to low phosphate stress of phosphate transporter family 1 (<i>pht1</i>)) genes in <i>solanum tuberosum</i> L.. Phyton-International Journal of Experimental Botany, 92(8), 2325-2348. https://doi.org/10.32604/phyton.2023.027642
Vancouver Style
Du Y, Bao Y, Zheng L, Tian Q, Zhang X. Biological analysis and response to low phosphate stress of phosphate transporter family 1 (<i>pht1</i>)) genes in <i>solanum tuberosum</i> L.. Phyton-Int J Exp Bot. 2023;92(8):2325-2348 https://doi.org/10.32604/phyton.2023.027642
IEEE Style
Y. Du, Y. Bao, L. Zheng, Q. Tian, and X. Zhang "Biological Analysis and Response to Low Phosphate Stress of Phosphate Transporter Family 1 (<i>PHT1</i>)) Genes in <i>Solanum tuberosum</i> L.," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2325-2348. 2023. https://doi.org/10.32604/phyton.2023.027642