Open Access
ARTICLE
TCD5 Enhances the Photosynthesis Capacity, Increases the Panicle Number and the Yield in Rice
Jing Yang1,2, Yufeng Wang2, Zhanghua Hu3, Xiaoping Chen4, Yanjun Dong5,*, Sheng Teng1,*
1 Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, China
2 CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China
3 Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
4 Agrotechnical Extension Station in Tiantai County, Taizhou, 317200, China
5 College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China
* Corresponding Authors: Yanjun Dong. Email: ; Sheng Teng. Email:
(This article belongs to the Special Issue: High-Yield Rice Physiology & Genetics)
Phyton-International Journal of Experimental Botany 2023, 92(9), 2649-2663. https://doi.org/10.32604/phyton.2023.030710
Received 19 April 2023; Accepted 19 May 2023; Issue published 28 July 2023
Abstract
Improvement of photosynthetic efficiency is a major approach to increase crop yield potential. Previously, we cloned a gene encoding the chloroplast-located putative monooxygenase
TCD5, which is essential in plastid development under low temperature in rice (
Oryza sativa L.). In this study, the effects of
TCD5 on the photosynthesis and the yields were investigated in rice. Two sets of genetic materials with three levels of
TCD5 expression, including
tcd5 mutant or
TCD5 RNAi transgenic lines and
TCD5 over-expression transgenic lines in Jiahua1 and Nipponbare backgrounds, were used in the field trails of multi-locations and multi-years. TCD5 positively affected the panicle number and the yield at dosage. Compared with the wild-types, the panicle numbers were 12.4%–14.6% less in
tcd5 mutant and 8.3%–38.6% less in
TCD5 RNAi lines, but 26.2%–61.8% more in
TCD5 over-expression lines. The grain yields per plant were 9.1%–18.4% less in
tcd5 mutant and 14.3%–56.7% less in TCD5 RNAi lines, but 6.9%–56.5% more in
TCD5 over-expression lines. The measurements of net photosynthetic rate indicated that mutation or knock down of
TCD5 decreased the net photosynthetic rate by 10.4% and 15.6%, respectively, while increasing it by 8.9% and 8.7% in the
TCD5 over-expression lines in Jiahua1 and Nipponbare backgrounds, respectively. Accordingly, the measurements of chlorophyll fluorescence parameters showed that the electron transport rate and quantum yield decreased in
tcd5 mutant or
TCD5 RNAi lines but increased in
TCD5 over-expression lines, both in Jiahua1 and Nipponbare backgrounds. IP-MS screening revealed that TCD5 interacts with 29 chloroplast proteins involved in chlorophyll synthesis, photo-reactions of the photosynthesis, carbon assimilation and metabolism, energy metabolism, redox balance, protein synthesis and transportation. Two TCD5 interacted proteins, D1 and FBA were effective targets for improving photosynthesis. These results suggest a potentially new strategy for increasing rice yield by enhancing photosynthesis.
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Cite This Article
APA Style
Yang, J., Wang, Y., Hu, Z., Chen, X., Dong, Y. et al. (2023). TCD5 enhances the photosynthesis capacity, increases the panicle number and the yield in rice. Phyton-International Journal of Experimental Botany, 92(9), 2649-2663. https://doi.org/10.32604/phyton.2023.030710
Vancouver Style
Yang J, Wang Y, Hu Z, Chen X, Dong Y, Teng S. TCD5 enhances the photosynthesis capacity, increases the panicle number and the yield in rice. Phyton-Int J Exp Bot. 2023;92(9):2649-2663 https://doi.org/10.32604/phyton.2023.030710
IEEE Style
J. Yang, Y. Wang, Z. Hu, X. Chen, Y. Dong, and S. Teng "TCD5 Enhances the Photosynthesis Capacity, Increases the Panicle Number and the Yield in Rice," Phyton-Int. J. Exp. Bot., vol. 92, no. 9, pp. 2649-2663. 2023. https://doi.org/10.32604/phyton.2023.030710